SMARCB1 anticorps (AA 257-359)

N° du produit ABIN968742

Détail du produit anti-SMARCB1 anticorps

Antigène: SMARCB1 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily B, Member 1 (SMARCB1))

Épitope: AA 257-359

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SMARCB1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Réactivité croisée: Rat (Rattus), Humain

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Mouse BAF47 aa. 257-359

Clone: 25-BAF47

Isotype: IgG2a

Information d'application

Commentaires:

Related Products: ABIN968540, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-SMARCB1 (ABIN968742)

Guidi, Sands, Zambrowicz, Turner, Demers, Webster, Smith, Imbalzano, Jones: "Disruption of Ini1 leads to peri-implantation lethality and tumorigenesis in mice." dans: Molecular and cellular biology, Vol. 21, Issue 10, pp. 3598-603, (2001) (PubMed).

Bruder, Dumanski, Kedra: "The mouse ortholog of the human SMARCB1 gene encodes two splice forms." dans: Biochemical and biophysical research communications, Vol. 257, Issue 3, pp. 886-90, (1999) (PubMed).

Kalpana, Marmon, Wang, Crabtree, Goff: "Binding and stimulation of HIV-1 integrase by a human homolog of yeast transcription factor SNF5." dans: Science (New York, N.Y.), Vol. 266, Issue 5193, pp. 2002-6, (1995) (PubMed).

Détails sur SMARCB1

Antigène: SMARCB1 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily B, Member 1 (SMARCB1))

Autre désignation: BAF47 (SMARCB1 Produits)

Synonymes: anticorps SMARCB1, anticorps BAF47, anticorps INI1, anticorps MRD15, anticorps RDT, anticorps RTPS1, anticorps SNF5, anticorps SNF5L1, anticorps Sfh1p, anticorps Snr1, anticorps hSNFS, anticorps AU020204, anticorps Baf47, anticorps Ini1, anticorps SNF5/INI1, anticorps Snf5, anticorps fc51b01, anticorps smarcb1, anticorps wu:fc51b01, anticorps SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1, anticorps SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1 S homeolog, anticorps SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1b, anticorps SMARCB1, anticorps Smarcb1, anticorps smarcb1.S, anticorps smarcb1b

Sujet: SWI/SNF complexes facilitate gene activation and transcription factor binding by altering repressive chromatin structures in an ATP-dependent manner. In mammals, SWI/SNF complexes are present in mutliple forms that include 9-12 BRG1-associated factors (BAFs) per complex. These BAF proteins range in molecular weight from 47 to 250 kDa. BAF47/SNF5 (SMARCB1/Ini1) complexes with BRG1- and BRM-containing SWI/SNF complexes. BAF47/SNF5 mRNA is widely expressed in cell lines and tissues, and BAF47/SNF5 gene is mutated in many human tumors. The latter is indicative of a tumor suppressor role for BAF47/SNF5. In addition, mice deficient for BAF47 die early in embryogenesis, while BAF47/SNF5 heterozygous mice display a variety of tumors in the soft tissues of the head and neck. BAF47/SNF5 also binds the HIV-1 integrase and stimulates integrase-mediated DNA joining activity. Thus, BAF47/SNF5 is a component of SWI/SNF complexes that may be critical for normal development and tumor suppression, but may also be a protein utilized for viral DNA integration into host DNA. This antibody is routinely tested by western blot analysis.
Synonyms: SNF5

Poids moléculaire: 47 kDa