CREB1 anticorps (pSer133)

N° du produit ABIN97863

N° du produit (Fournisseur): 600-401-270

Cet anticorps anti-CREB1 est un anticorps Lapin Polyclonal détectant CREB1 dans WB, IHC et ELISA. Adapté pour Humain, Souris et Rat. Ce Primary Antibody a été cité dans 1 publication.

Aperçu rapide pour CREB1 anticorps (pSer133) (ABIN97863)

Antigène: CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CREB1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Détail du produit anti-CREB1 anticorps

Épitope: pSer133

N° du produit (Fournisseur): 600-401-270

Fournisseur: Rockland

Fonction: CREB phospho S133 Antibody

Attributs du produit: Synonyms: rabbit anti-CREB pS133 Antibody, Cyclic AMP-responsive element-binding protein 1, Active transcription factor CREB antibody, cAMP responsive element binding protein 1 antibody, CREB 1 antibody, CREB-1, CREB1 antibody, MGC9284 antibody, Transactivator protein antibody

Purification: Anti-CREB pS133 was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first sequentially pre-adsorbed against a control E.

Stérilité: Sterile filtered

Immunogène:

Immunogen: CREB phospho peptide corresponding to amino acid residues 122-147 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 20 μg/mL

Application Note: Anti-CREB pS133 antibody reacts with phosphorylated human CREB at pS133 and shows minimal reactivity by western blot with non-phosphorylated CREB and minimal reactivity (1 % ) by ELISA against the non-phosphorylated form of the immunizing peptide.  This antibody was assayed against a variety of tissues including fibroblasts and B-cell lysates.  Bands of 46 and 43 kDa corresponding to phosphorylated CREB are observed in western blots.  Anti-CREB pS133 is suitable for the detection by immunoblot of phosphorylated human, mouse and rat CREB.  No cross-reactivity occurs with non-phosphorylated CREB.  For immunohistochemistry, formalin fixed, paraffin embedded human tissue shows moderate to strong nuclear staining in a variety of cells with minimal background staining.  Although not tested this antibody is likely functional by FACS and immunoprecipitation.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:1,000 - 1:6,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.064 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-CREB1 (ABIN97863)

Philp, Chen, Lan, Meyer, Murphy, Knapp, Olfert, McCurdy, Marcotte, Hogan, Baar, Schenk et al.: "Sirtuin 1 (SIRT1) deacetylase activity is not required for mitochondrial biogenesis or peroxisome proliferator-activated receptor-gamma coactivator-1alpha (PGC-1alpha) deacetylation following ..." dans: The Journal of biological chemistry, Vol. 286, Issue 35, pp. 30561-70, (2011) (PubMed).

Détails sur CREB1

Antigène: CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))

Autre désignation: CREB1

Sujet: Background: The CREB (Cyclic AMP-response-element-binding-protein 1) gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element (CRE element TGANNTCA), an octameric palindrome. Phosphorylation by cAMP-dependent protein kinase (PKA) at serine-119 is required for interaction with DNA and phosphorylation at serine-133 allows CREB to interact with CBP (CREB binding protein) leading to interaction with RNA polymerase II.  Alternate splicing of this gene results in two transcript variants encoding different isoforms.

ID gène: 1385

UniProt: P16220

Pathways: Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Cell Size, Toll-Like Receptors Cascades, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Positive Regulation of fat Cell Differentiation