ZAP70 anticorps (Internal Region)

N° du produit ABIN987274

Cet anticorps Chèvre Polyclonal détecte spécifiquement ZAP70 dans WB, ELISA et IP. Il présente une réactivité envers Humain.

Aperçu rapide pour ZAP70 anticorps (Internal Region) (ABIN987274)

Antigène: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp ZAP70 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Classe de qualité: Verified

Détail du produit anti-ZAP70 anticorps

Épitope: Internal Region

Fonction: ZAP70

Séquence: SDGYTPEPAR ITSPD

Specificité: This antibody is expected to recognize isoform 1 (NP_001070.2) only.

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-SDGYTPEPARITSPD, from the internal region of the protein sequence according to NP_001070.2.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 70 kDa band observed in lysates of cell lines Jurkat and Molt4 (calculated MW of 69.9 kDa according to NP_001070.2). Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Commentaires:

Immunoprecipitation:This antibody was deemed fit for IP under native conditions (observations from customer).

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (201

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur ZAP70

Antigène: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Autre désignation: ZAP70

Sujet: ZAP70, zeta-chain (TCR) associated protein kinase 70 kDa, FLJ17670, FLJ17679, SRK, STD, TZK, ZAP-70, 70 kDa zeta-associated protein, syk-related tyrosine kinase, tyrosine-protein kinase ZAP-70, zeta-chain associated protein kinase, 70kD

ID gène: 7535, 22637, 301348

NCBI Accession: NP_001070

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway