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Independently validated

Here you can find our antibodies which have been validated through our Independent Validation Initiative(IVI).

The IVI is centered around core values of transparency, reproducibility, scientific integrity. We are committed to improving the antibody and antibody market by providing independently collected, scientifically sound, and properly documented data for the products that we sell, which is why we set very high standards for independent validation data. Our support can answer further questions via phone, live chat or email.

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239 Résultats :

LEF1 anticorps (AA 100-399)

LEF1 Reactivité: Humain WB Hôte: Lapin Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (AA 100-399) antibody (ABIN1680678)

Cleavage Under Targets and Release Using Nuclease validation image for anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (AA 100-399) antibody (ABIN1680678)

Western blot analysis of extracts of various cell lines, using LEF1 antibody (ABIN1680678, ABIN5663809, ABIN5663811 and ABIN6214021) at 1:1000 dilution.

Immunofluorescence analysis of rat thymus cells using LEF1 Rabbit pAb (ABIN1680678, ABIN5663809, ABIN5663811 and ABIN6214021) at dilution of 1:25 (40x lens).

Alignment tracks from CUT&RUN targeting LEF1 in HEK293T cells using anti-LEF1 antibody ABIN1680678 (1).

Library profiles comparing fragment size distributions on an E-Gel EX 2% agarose gel (Thermo Fisher).

Immunoprecipitation analysis of 300 μg extracts of Jurkat cells using 3 μg LEF1 antibody (ABIN1680678, ABIN5663809, ABIN5663811 and ABIN6214021).

Immunofluorescence analysis of mouse thymus cells using LEF1 Rabbit pAb (ABIN1680678, ABIN5663809, ABIN5663811 and ABIN6214021) at dilution of 1:25 (40x lens).

8 images

1 reference

Independent Validation

1 validation

N° du produit ABIN5663810

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Collagen IV anticorps

COL4 Reactivité: Humain, Boeuf (Vache), Mammifères WB, ELISA, IHC, IP Hôte: Lapin Polyclonal unconjugated

Immunohistochemistry validation image for anti-Collagen, Type IV (COL4) antibody (ABIN5596835)

Immunohistochemistry validation image for anti-Collagen, Type IV (COL4) antibody (ABIN5596835)

Immunohistochemistry validation image for anti-Collagen, Type IV (COL4) antibody (ABIN5596835)

anti collagen IV antibody (600-401-106 Lot 25440, 1:400, 45 min RT) showed strong staining in FFPE sections of human kidney (Left) with strong red staining observed in glomeruli; and liver (Right) with strong staining in sinusoids.

SMFC tracking in metamorphosed newt limb regeneration.

SMFC tracking in larval newt limb regeneration.

Lamina lucida and lamina densa regenerate before new ECM deposition.

SMFC tracking in larval newt limb regeneration.

SMFC tracking in metamorphosed newt limb regeneration.

SMFC tracking in larval newt limb regeneration.

SMFC tracking in larval newt limb regeneration.

SMFC tracking in metamorphosed newt limb regeneration.

12 images

8 références

Independent Validation

1 validation

N° du produit ABIN5596835

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COL3 anticorps

COL3 Reactivité: Humain, Boeuf (Vache), Mammifères WB, ELISA, IHC, IP Hôte: Lapin Polyclonal unconjugated

Immunohistochemistry validation image for anti-Collagen, Type III (COL3) antibody (ABIN5596830)

Immunohistochemistry validation image for anti-Collagen, Type III (COL3) antibody (ABIN5596830)

Immunohistochemistry validation image for anti-Collagen, Type III (COL3) antibody (ABIN5596830)

Immunohistochemistry validation image for anti-Collagen, Type III (COL3) antibody (ABIN5596830)

anti collagen III antibody (600-401-105 Lot 26016, 1:400, 45 min RT) showed strong staining in FFPE sections of human skin(left, dermis) with moderate to strong red staining and testis (right) where strong staining was observed within connective tissue between seminiferous tubules.

Western Blot of Rabbit Anti-COLLAGEN III Antibody Lane 1: Human Collagen III Load: 100 ng per lane Primary antibody: Collagen III Antibody at 1:1000 o/n at 4°C Secondary antibody: 649 Goat anti-rabbit at 1:20,000 for 30 min at RT Block: ABIN925618 for 30 min at RT Predicted/Observed size: 138 kDa, 138 kDa

Immunohistochemistry of Rabbit Anti-collagen type III antibody.

(a) Typical electropherogram of α (I) subunits, oligomers β/γ (I), and collagen type I degradation products, released from healthy and burnt skin.

Typical electropherogram of α (III) subunits, oligomers β/γ (III), and collagen type III degradation products, released from healthy and burned skin.

9 images

5 références

Independent Validation

1 validation

N° du produit ABIN5596830

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Fiche de données

CBP anticorps (C-Term)

KO Validated CREBBP Reactivité: Humain WB, IF, IP, IHC (p), ICC, ChIP Hôte: Lapin Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-CREB Binding Protein (CREBBP) (C-Term) antibody (ABIN2854987)

Cleavage Under Targets and Release Using Nuclease validation image for anti-CREB Binding Protein (CREBBP) (C-Term) antibody (ABIN2854987)

WB Image Sample (30 ug of whole cell lysate) A: H1299 5% SDS PAGE antibody diluted at 1:1000

IHC-P Image CBP antibody [C3], C-term detects CBP protein at nucleus on mouse skin by immunohistochemical analysis.

IHC-P Image CBP antibody [C3], C-term detects CBP protein at nucleus on human breast carcinoma by immunohistochemical analysis.

IP Image CBP antibody immunoprecipitates CBP protein in IP experiments.

ChIP Image CREBBP antibody immunoprecipitates CREBBP protein-DNA complex in ChIP experiments.

IHC-P Image CBP antibody [C3], C-term detects CBP protein at nucleus on human cervical carcinoma by immunohistochemical analysis.

IHC-P Image CBP antibody [C3], C-term detects CBP protein at nucleus on mouse muscle by immunohistochemical analysis.

IHC-P Image CBP antibody [C3], C-term detects CBP protein at nucleus on mouse fore brain by immunohistochemical analysis.

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CBP , antibody at 1:200 dilution.

11 images

1 reference

Independent Validation

1 validation

N° du produit ABIN2854987

Fiche technique

Fiche de données

CTNNB1 anticorps (N-Term)

KO Validated CTNNB1 Reactivité: Humain WB, IHC, IF, FACS, IHC (p), IP, ICC, IHC (fro), ChIP, IHC (wm), CUT&RUN Hôte: Lapin Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1) (N-Term) antibody (ABIN2855042)

Cleavage Under Targets and Release Using Nuclease validation image for anti-Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1) (N-Term) antibody (ABIN2855042)

WB Image beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein by western blot analysis.

WB Image Sample (30 ug of whole cell lysate) A: HeLa 7.

IHC-P Image beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at membrane on mouse urinary bladder by immunohistochemical analysis.

WB Image Sample (50 ug of whole cell lysate) A: mouse brain 7.

IHC-P Image beta Catenin antibody [N1N2-2] detects beta Catenin protein at cell membrane in mouse colon by immunohistochemical analysis.

ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using beta Catenin, antibody (Green) at 1:200 dilution.

WB Image beta Catenin antibody [N1N2-2], N-term detects CTNNB1 protein by Western blot analysis.

IP Image Immunoprecipitation of beta Catenin protein from HeLa whole cell extracts using 5 μg of beta Catenin antibody, Western blot analysis was performed using beta Catenin antibody, EasyBlot anti-Rabbit IgG was used as a secondary reagent.

IHC-P Image beta Catenin antibody [N1N2-2] detects beta Catenin protein at cell membrane in mouse colon by immunohistochemical analysis.

IHC-P Image Immunohistochemical analysis of paraffin-embedded U373 xenograft, using beta Catenin, antibody at 1:500 dilution.

Effects of MCME on migration-related proteins in CL1-0 and CL1-5 cells.

Expression analysis of Wnt pathway genes in human OS cellsWestern blot analysis of active β-catenin, total β-catenin and β-actin proteins in human OS cell lines (143B, Saos-2, SJSA-1 , U-2 OS, MG-63) with human breast cancer cell high-level-expression controls (MCF7, MDA-MB-231) and additional human mesenchymal stem cell low-level-expression controls (hMSC).

SGSM2 silencing affected T47D cell adhesion and cell migration.

Interaction between SGSM2 and E-cadherin was verified by co-immunoprecipitation and immunofluorescence staining assays.

16 images

8 références

Independent Validation

1 validation

N° du produit ABIN2855042

Fiche technique

Fiche de données

HDAC1 anticorps

KO Validated HDAC1 Reactivité: Humain WB, IF, IHC (p), IP, ICC, ChIP, IHC (fro), IHC (wm) Hôte: Lapin Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Histone Deacetylase 1 (HDAC1) antibody (ABIN2854776)

Cleavage Under Targets and Release Using Nuclease validation image for anti-Histone Deacetylase 1 (HDAC1) antibody (ABIN2854776)

WB Image HDAC1 antibody detects HDAC1 protein by western blot analysis.

Library profiles comparing fragment size distributions on an E-Gel EX 2% agarose gel (Thermo Fisher).

IHC-P Image Immunohistochemical analysis of paraffin-embedded human testis, using HDAC1, antibody(10 μg/ml).

IHC-P Image HDAC1 antibody detects HDAC1 protein at nucleus on mouse colon by immunohistochemical analysis.

ICC/IF Image HDAC1 antibody detects HDAC1 protein at nucleus by immunofluorescent analysis.

IHC-P Image HDAC1 antibody detects HDAC1 protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis.

ChIP Image HDAC1 antibody immunoprecipitates HDAC1 protein-DNA in ChIP experiments.

IHC-P Image HDAC1 antibody detects HDAC1 protein at nucleus in human lung adenocarcinoma by immunohistochemical analysis.

IHC-P Image HDAC1 antibody detects HDAC1 protein at nucleus on mouse liver by immunohistochemical analysis.

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed A431, using HDAC1, antibody at 1:200 dilution.

IP Image HDAC1antibody immunoprecipitates HDAC1 protein in IP experiments.

HDAC1/2 depletion in adult Schwann cells leads to motor and sensory dysfunction in peripheral nerves.

HDAC1/2 depletion in adult Schwann cells leads to motor and sensory dysfunction in peripheral nerves.

SAHA affects the expression of suvivin and XIAP at the transcriptional level.

HDAC1/2 are robustly upregulated in SCs after lesion.

HDAC1/2 are robustly upregulated in SCs after lesion.

Overexpression of peptidase inhibitor 16 (PI16) attenuates angiotensin II (Ang II)-induced fibrotic gene expression and histone 3 modification in NRCFs.

The effects of peptidase inhibitor 16 (PI16) on newborn rat cardiac fibroblasts (NRCFs) are partially rescued by overexpression of HDAC1.

The effects of peptidase inhibitor 16 (PI16) on newborn rat cardiac fibroblasts (NRCFs) are partially rescued by overexpression of HDAC1.

Alignment tracks from CUT&RUN targeting HDAC1 in Mouse fore limb (11.

22 images

7 références

Independent Validation

1 validation

N° du produit ABIN2854776

Fiche technique

Fiche de données

STAT3 anticorps (C-Term)

KO Validated STAT3 Reactivité: Humain WB, IF, IHC (p), ICC Hôte: Lapin Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (C-Term) antibody (ABIN2855865)

Cleavage Under Targets and Release Using Nuclease validation image for anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (C-Term) antibody (ABIN2855865)

WB Image Sample (20 ug of whole cell lysate) A: human ESC 7.

WB Image Sample(30 ug whole cell lysate) A: 293T B: A431 , C: H1299 D: HeLa S3 , E: Hep G2 , F: MOLT4 , 7.

WB Image STAT3 antibody [C3], C-term detects STAT3 protein by Western blot analysis.

WB Image STAT3 antibody [C3], C-term detects STAT3 protein by Western blot analysis.

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using STAT3, antibody at 1:200 dilution.

Effects of treatment with antrodan only, cisplatin only, and a combination of both on protein expression of MMP-2 and MMP-9, and on the phosphorylation and protein expression of signal transducer and activator of transcription 3 (STAT3), extracellular signal-regulated kinase 1 (ERK-1), c-Jun N-terminal kinase 1 (JNK1), JNK2, and p38 in lung tissues of C57BL/6 mice.

Effects of treatment with antrodan only, cisplatin only, and a combination of both on protein expression of MMP-2 and MMP-9, and on the phosphorylation and protein expression of STAT3, ERK1, JNK1, JNK2, and p38 in liver tissues of C57BL/6 mice.

MLN8237 treatment resulted in abnormal activation of Akt/Stat3 pathway.

10 images

1 reference

Independent Validation

1 validation

N° du produit ABIN2855865

Fiche technique

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Calretinin anticorps

CALB2 Reactivité: Humain WB, IHC (p), IF, ICC, IHC (fro) Hôte: Lapin Polyclonal unconjugated

Immunohistochemistry validation image for anti-Calbindin 2 (CALB2) antibody (ABIN2855596)

IHC-P Image Immunohistochemical analysis of paraffin-embedded Mouse retina, using Calretinin, antibody at 1:250 dilution.

WB Image Calretinin antibody detects CALB2 protein by Western blot analysis.

WB Image Sample (30 ug of whole cell lysate) A: A431 , 12% SDS PAGE Calretinin antibody antibody diluted at 1:1000

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed A431, using Calretinin , antibody at 1:200 dilution.

WB Image Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE antibody diluted at 1:1000

IHC-P Image Calretinin antibody detects Calretinin protein at cytosol on mouse fore brain by immunohistochemical analysis.

7 images

1 reference

Independent Validation

1 validation

N° du produit ABIN2855596

Fiche technique

Fiche de données

TCF7L1 anticorps (N-Term)

TCF7L1 Reactivité: Humain WB, ChIP-seq, ChIP, CUT&RUN Hôte: Lapin Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Transcription Factor 7-Like 1 (T-Cell Specific, HMG-Box) (TCF7L1) (N-Term) antibody (ABIN6972849)

Cleavage Under Targets and Release Using Nuclease validation image for anti-Transcription Factor 7-Like 1 (T-Cell Specific, HMG-Box) (TCF7L1) (N-Term) antibody (ABIN6972849)

Western blot of TCF7L1 / TCF3 antibody.

Library profiles comparing fragment size distributions on an E-Gel EX 2% agarose gel (Thermo Fisher).

TCF7L1 / TCF3 antibody (pAb) tested by ChIP-Seq.

Alignment tracks from CUT&RUN targeting TCF7L1 in HEK293T cells using anti-TCF7L1 antibody ABIN6972849 (1).

6 images

1 reference

Independent Validation

1 validation

N° du produit ABIN6972849

Fiche technique

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NFKB1 anticorps (AA 740-964)

NFKB1 Reactivité: Humain WB, IHC, IF Hôte: Lapin Polyclonal unconjugated

Immunofluorescence validation image for anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1) (AA 740-964) antibody (ABIN6144571)

Western blot analysis of extracts of various cell lines, using NFKB1 antibody.

Immunofluorescence analysis of U2OS cells using NFKB1 Rabbit pAb (ABIN6130581, ABIN6144571, ABIN6144573 and ABIN7101802) at dilution of 1:50 (40x lens).

Immunohistochemistry of paraffin-embedded mouse lung using NFKB1 antibody.

Immunofluorescence analysis of A549 cells using NFKB1 antibody.

Immunofluorescence analysis of A-549 cells using NFKB1 Rabbit pAb (ABIN6130581, ABIN6144571, ABIN6144573 and ABIN7101802) at dilution of 1:50 (40x lens).

Immunohistochemistry of paraffin-embedded rat lung using NFKB1 Rabbit pAb (ABIN6130581, ABIN6144571, ABIN6144573 and ABIN7101802) at dilution of 1:50 (40x lens).

Immunofluorescence analysis of NIH/3T3 cells using NFKB1 Rabbit pAb (ABIN6130581, ABIN6144571, ABIN6144573 and ABIN7101802) at dilution of 1:50 (40x lens).

8 images

2 références

Independent Validation

1 validation

N° du produit ABIN6144571

Fiche technique

Fiche de données

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