IL-1 beta Kit ELISA

N° du produit ABIN1115634

Détail du produit IL-1 beta Kit ELISA

Antigène: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 7.81 pg/mL - 500 pg/mL

Seuil minimal de détection: 7.81 pg/mL

Application: ELISA

Fonction: The kit is a sandwich enzyme immunoassay technique for the in vitro quantitative measurement in various sample types.

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This kit recognizes natural and recombinantMouseIL-1β. No significant cross-reactivity or interference between MouseIL-1β and analogues was observed. Note: Limited by existing techniques, cross reaction may still exist, as it is impossible for us to complete the cross-reactivity detection between MouseIL-1β and all the analogues.

Réactivité croisée (Details): No significant cross-reactivity or interference between mouse Interleukin 1beta and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between Human bFGF and all the analogues, therefore, cross reaction may still exist.

Sensibilité: 4.69 pg/mL

Ingrédients:

• Pre-coated, ready to use 96-well strip plate, flat buttom
• Plate sealer for 96 wells
• Reference Standard
• Reference Standard & Sample Diluent
• Biotinylated Detection Antibody (100 x concentrate)
• HRP Conjugate (100 x concentrate)
• Biotinylated Detection Antibody Diluent
• HRP Conjugate Diluent
• Substrate Reagent
• Stop Solution
• Wash Buffer (25 x concentrate)
• Instruction manual

Information d'application

Indications d'application: ELISA Plate: The just opened ELISA Plate may appear water-like substance, which is normal and will not have any impact on the experiment results.
Add Sample: The interval of sample adding between the first well and the last well should not be too long, otherwise will cause different pre-incubation time, which will significantly affect the experiment’s accuracy and repeatability. For each step in the procedure, total dispensing time for addition of reagents or samples to the assay plate should not exceed 10 minutes. Parallel measure ment is recommended.
Incubation: To prevent evaporation and ensure accurate results, proper adhesion of plate sealers during incubation steps is necessary. Do not allow wells to sit uncovered for extended periods between incubation steps. Do not let the strips dry at any time during the assay. Strict compliance with the given incubation time and temperature.
Washing: The wash procedure is critical. Insufficient washing will result in poor precision and falsely elevated absorbance readings. Residual liquid in the reaction wells should be pat dry against absorbent paper in the washing process. But don’t put absorbent paper into reaction wells directly. Note that clear the residual liquid and fingerprint in the bottom before measurement, so as not to affect the micro-titer plate reader.
Reagent Preparation: As the volume of Detection Ab and HRP Conjugate is very small, liquid may adhere to the tube wall or tube cap when being transported. You better hand-throw it or centrifugal it for 1 minute at 1000rpm. Please pipette the solution for 4-5 times before pippeting. Please carefully reconstitute Standards, working solutions of Detection Ab and HRP Conjugate according to the instructions. To minimize imprecision caused by pipetting, ensure that pipettors are calibrated. It is recommended to suck more than 10μL for once pipetting. Do not reuse standard solution, working solution of Detection Ab and HRP Conjugate, which have been diluted. If you need to use standard repeatedly, you can divide the standard into small pack according to the amount of each assay, keep them at -20°C to -80°C and avoid repeated freezing and thawing.
Reaction Time Control: Please control reaction time strictly following this product description!
Substrate: Substrate Solution is easily contaminated. Please protect it from light.Stop Solution: As it is an acid solution, please pay attention to the protection of your eyes, hands, face and clothes when using this solution.
Mixing: You’d better use micro-oscillator at the lowest frequency, as sufficient and gentle mixing is particularly important to reaction result. If there is no micro-oscillator available, you can knock the ELISA plate frame gently with your finger before reaction.
Security: Please wear lab coats and latex gloves for protection. Especially detecting samples of blood or other body fluid, please perform following the national security columns of biological laboratories.
Do not use component from different batches of kit(washing buffer and stop solution can be an exception)
To avoid cross-contamination, change pipette tips between adding of each standard level, between sample adding, and between reagent adding. Also, use separate reservoirs for each reagent. Otherwise, the results will be inaccurate!

Commentaires:

Information on standard material:
The formulation of the standard is 0.01 M PBS. The standard contains additives (1 % BSA).

Information on reagents:
Reagents include 1 M SO₂. Azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials are not used.

Information on antibodies:
The provided antibodies and their host vary in different kits. All antibodies are affinity purified

The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.

Volume d'échantillon: 100 µL

Plaque: Pre-coated

Protocole:

1. Add 100 µL standard or sample to each well. Incubate for 90 min at 37 °C.
2. Remove the liquid. Add 100 µL Biotinylated Detection Antibody. Incubate for 1 hour at 37 °C.
3. Aspirate and wash 3 times.
4. Add 100 µL HRP Conjugate. Incubate for 30 min at 37 °C.
5. Aspirate and wash 5 times.
6. Add 90 µL Substrate Reagent. Incubate for 15 min at 37 °C.
7. Add 50 µL Stop Solution. Read at 450 nm immediately.
8. Calculation of results.

Préparation des réactifs:

1. Bring all reagents to room temperature (18~25 °C) before use. Follow the Microplate reader manual for set-up and preheat it for 15 min before OD measurement.
2. Wash Buffer: Dilute 30 mL of Concentrated Wash Buffer with 720 mL of deionized or distilled water to prepare 750 mL of Wash Buffer.Note: if crystals have formed in the concentrate, warm it in a 40 °C water bath and mix it gently until the crystals have completely dissolved
3. Standard working solution: Centrifuge the standard at 10,000xg for 1 min. Add 1.0 mL of Reference Standard &Sample Diluent, let it stand for 10 min and invert it gently several times. After it dissolves fully, mix it thoroughly with a pipette. This reconstitution produces a working solution of 500 pg/mL. Then make serial dilutions as needed. The recommended dilution gradient is as follows: 500, 250, 125, 62.5, 31.25, 15.63, 7.81, 0 pg/mL. Dilution method: Take 7 EP tubes, add 500 μLof Reference Standard & Sample Diluent to each tube. Pipette 500 μLof the 500 pg/mL working solution to the first tube and mix up to produce a 250 pg/mL working solution. Pipette 500 μLof the solution from the former tube into the latter one according to these steps. The illustration below is for reference. Note: the last tube is regarded as a blank. Don't pipette solution into it from the former tube.
4. Biotinylated Detection Antibody working solution: Calculate the required amount before the experiment (100 μL/well). In preparation, slightly more than calculated should be prepared. Centrifuge the stock tube before use, dilute the 100x Concentrated Biotinylated Detection Antibody to 1xworking solution with Biotinylated Detection Antibody Diluent.
5. Concentrated HRP Conjugate working solution: Calculate the required amount before the experiment (100 μL/well). In preparation, slightly more than calculated should be prepared. Dilute the 100x Concentrated HRP Conjugate to 1x working solution with Concentrated HRP Conjugate Diluent.

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: All the reagents in the kit should be stored according to the labels on vials. Unused wells should be returned to the foil pouch with the desiccant pack and resealed along entire edge of zip-seal. Substrate Reagent shouldn't be kept at -20 °C (Check!). Exposure of reagents to strong light should be avoided in the process of incubation and storage. All the taps of reagents should be tightened to prevent evaporation and microbial contamination. If not to store reagents according to above suggestions, erroneous results may occur.

Stock: 4 °C/-20 °C

Stockage commentaire: The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the conditions since the kit is received.

Références pour IL-1 beta Kit ELISA (ABIN1115634)

Wang, Gao, Song, Tang, Wang, Que, Liu, Zhu, Chen, Yao, Xu, Li, Li: "The TIR/BB-loop mimetic AS-1 prevents non-alcoholic steatohepatitis and hepatic insulin resistance by inhibiting NLRP3-ASC inflammasome activation." dans: British journal of pharmacology, Vol. 174, Issue 12, pp. 1841-1856, (2018) (PubMed).

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Lin, Shao, Zeng, Zhao, Fang, Wang, Pan, Liu, Qi, Feng, Qiu, Zhang, Chen, Wang, Cai, Xian: "Blocking PI3K/AKT signaling inhibits bone sclerosis in subchondral bone and attenuates post-traumatic osteoarthritis." dans: Journal of cellular physiology, Vol. 233, Issue 8, pp. 6135-6147, (2018) (PubMed).

Xia, Yu, Ma, Tu, Zhai, Yang, Liu, Liu, Wu, Dan, You: "Yu Gan Long reduces rat liver fibrosis by blocking TGF-β1/Smad pathway and modulating the immunity." dans: Biomedicine & pharmacotherapy, Vol. 106, pp. 1332-1338, (2018) (PubMed).

Wang, Wang, Zhou, Qin, Chen: "The protective effect of formononetin on cognitive impairment in streptozotocin (STZ)-induced diabetic mice." dans: Biomedicine & pharmacotherapy, Vol. 106, pp. 1250-1257, (2018) (PubMed).

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Chen, Lin, Yeh, Hu, Sheu: "Propofol specifically suppresses IL-1β secretion but increases bacterial survival in Staphylococcus aureus-infected RAW264.7 cells." dans: Molecular and cellular biochemistry, Vol. 449, Issue 1-2, pp. 117-125, (2018) (PubMed).

Zhang, Zhang, Chen, Wang: "Oral supplementation with ursolic acid ameliorates sepsis-induced acute kidney injury in a mouse model by inhibiting oxidative stress and inflammatory responses." dans: Molecular medicine reports, Vol. 17, Issue 5, pp. 7142-7148, (2018) (PubMed).

Zhang, Wang, Guo, Zhu, Yan, Sun, Xu, Lin: "Molecular mechanisms of the analgesic action of Wu-tou Decoction on neuropathic pain in mice revealed using microarray and network analysis." dans: Acta pharmacologica Sinica, Vol. 39, Issue 6, pp. 988-997, (2018) (PubMed).

Hu, Luo, Alitongbieke, Chong, Xu, Xie, Chen, Zhang, Zhou, Wang, Ye, Cai, Zhang, Chen, Jiang, Fang, Yang, Liu, Diaz-Meco, Su, Zhou, Moscat, Lin, Zhang: "Celastrol-Induced Nur77 Interaction with TRAF2 Alleviates Inflammation by Promoting Mitochondrial Ubiquitination and Autophagy." dans: Molecular cell, Vol. 66, Issue 1, pp. 141-153.e6, (2017) (PubMed).

Hamuti, Li, Zhou, Aipire, Ma, Yang, Li: "Capparis spinosa Fruit Ethanol Extracts Exert Different Effects on the Maturation of Dendritic Cells." dans: Molecules (Basel, Switzerland), Vol. 22, Issue 1, (2017) (PubMed).

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Chen, Shen, Chen, Cao, Yang: "A Comparative Study of the Effects upon LPS Induced Macrophage RAW264.7 Inflammation in vitro of the Lipids of Hippocampus trimaculatus Leach." dans: Journal of oleo science, Vol. 64, Issue 12, pp. 1273-81, (2016) (PubMed).

Qi, Yin, Wang, Jiang, Wang, Ren, Su, Lei, Feng: "PGC-1α Silencing Compounds the Perturbation of Mitochondrial Function Caused by Mutant SOD1 in Skeletal Muscle of ALS Mouse Model." dans: Frontiers in aging neuroscience, Vol. 7, pp. 204, (2015) (PubMed).

Détail du antigène

Antigène: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Autre désignation: Interleukin 1beta (IL1B Produits)

Sujet: Synonyms: IL1B, IL1-BETA, IL1F2, catabolin

Pathways: Signalisation NF-kappaB, Interferon-gamma Pathway, Signalisation TLR, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome