Prolactin Kit ELISA

N° du produit ABIN1326881

Détail du produit Prolactin Kit ELISA

Antigène: Prolactin (PRL)

Reactivité: Rat, Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: The prolactin kit is based on a solid phase sandwich ELISA method. The samples and diluted biotinylated anti-prolactin antibodies are added to the wells coated with polyclonal antibody to prolactin. Prolactin in samples binds to the anti-prolactin polyclonal antibody on the well and the biotinylated antiprolactin antibody binds to prolactin. Unbound protein and biotin conjugate are washed off by wash buffer. Diluted horseradish peroxidase conjugated streptavidin is added to each wells and the streptavidin is allowed to bind to the biotin in the complex. Unbound enzyme conjugate is washed off. Upon the addition of the substrate, the intensity of color is proportional to the concentration of prolactin in the samples. A standard curve is prepared relating color intensity to the concentration of the prolactin.

Analytical Method: Quantitative

Information d'application

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Références pour Prolactin Kit ELISA (ABIN1326881)

Clarke, Russell, Findlay, Sastra, Anderson, Skinner, Atkins, Zajac, Davey: "A ROLE FOR THE CALCITONIN RECEPTOR TO LIMIT BONE LOSS DURING LACTATION IN FEMALE MICE BY INHIBITING OSTEOCYTIC OSTEOLYSIS." dans: Endocrinology, pp. en20151345, (2015) (PubMed).

Kahraman, Dirice, De Jesus, Hu, Kulkarni: "Maternal insulin resistance and transient hyperglycemia impact the metabolic and endocrine phenotypes of offspring." dans: American journal of physiology. Endocrinology and metabolism, Vol. 307, Issue 10, pp. E906-18, (2014) (PubMed).

Détail du antigène

Antigène: Prolactin (PRL)

Autre désignation: Prolactin (PRL Produits)

Synonymes: PRL Kit ELISA, prolactin Kit ELISA, PRLB Kit ELISA, PRLSD1 Kit ELISA, Prl1a1 Kit ELISA, Prol Kit ELISA, RATPRLSD1 Kit ELISA, RNPROL Kit ELISA, AV290867 Kit ELISA, prl Kit ELISA, prolactin Kit ELISA, prolactin, gene 2 L homeolog Kit ELISA, PROLACTIN protein Kit ELISA, prolactin-like Kit ELISA, PRL Kit ELISA, prl.2.L Kit ELISA, Prl Kit ELISA, LOC100136792 Kit ELISA, LOC100136580 Kit ELISA, PROLACTIN Kit ELISA, LOC101843376 Kit ELISA

Sujet: Prolactin (lactogenic hormone) is a single chain polypeptide hormone with a molecular weight of approximately 23,000 daltons. Prolactin is secreted from the anterior pituitary gland. Plasma and pituitary PRL levels are significantly greater in adult female than in male rats. This difference is thought to be brought about by ovarian steroids. A similar sex difference was found in pituitary PRL content in normal mice from 30 days of age. During and following pregnancy, prolactin, in association with other hormones, stimulates breast development and milk production. Hypersecretion of prolactin can be caused by pituitary tumors, hypothalamic diseases, hypothyroidm, renal failure, acute exercise and several medications.

Pathways: Signalistation JAK/STAT, Peptide Hormone Metabolism, Response to Growth Hormone Stimulus, Protein targeting to Nucleus