HDAC1 Kit ELISA

N° du produit ABIN1570266

Détail du produit HDAC1 Kit ELISA

Antigène: HDAC1 (Histone Deacetylase 1 (HDAC1))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.781 ng/mL - 50 ng/mL

Seuil minimal de détection: 0.781 ng/mL

Application: ELISA

Fonction: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of HDAC1 in rat tissue homogenates, cell lysates and other biological fluids.

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of this index.

Réactivité croisée (Details): No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.

Sensibilité: 0.28 ng/mL

Ingrédients:

• Pre-coated, ready to use 96-well strip plate
• Standard (freeze dried)
• Standard Diluent
• Detection Reagent A
• Detection Reagent B
• Assay Diluent A
• Assay Diluent B
• TMB
• Stop Solution
• Wash Buffer (30X)
• Plate sealer for 96 wells
• Instruction manual

Matériel non inclus:

1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Volume d'échantillon: 100 μL

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50µL Stop Solution. Read at 450nm immediately.

Procédure de l'essai:

The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste:

• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
• CV(%) = SD/meanX100
• Intra-assay: CV<10%
• Inter-assay: CV<12%

Restrictions: For Research Use only

Stockage

Précaution d'utilisation: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Conseil sur la manipulation: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.

Stock: 4 °C,-20 °C

Stockage commentaire: The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.

Date de péremption: 12 months

Détail du antigène

Antigène: HDAC1 (Histone Deacetylase 1 (HDAC1))

Autre désignation: HDAC1 (HDAC1 Produits)

Synonymes: GON-10 Kit ELISA, HD1 Kit ELISA, RPD3 Kit ELISA, RPD3L1 Kit ELISA, CG7471 Kit ELISA, DHDAC1 Kit ELISA, DRpd3 Kit ELISA, DmHDAC1 Kit ELISA, Dmel\\CG7471 Kit ELISA, E(var)3-64BC Kit ELISA, HDAC Kit ELISA, HDAC-1 Kit ELISA, HDAC1 Kit ELISA, Hdac1 Kit ELISA, Rpd3/HDAC Kit ELISA, Su(var)3-26 Kit ELISA, Su(var)326 Kit ELISA, Su(var)328 Kit ELISA, dHDAC-1 Kit ELISA, dHDAC1 Kit ELISA, dRPD3 Kit ELISA, dRpd3 Kit ELISA, dmHDA401 Kit ELISA, drpd3 Kit ELISA, hdac1 Kit ELISA, l(3)04556 Kit ELISA, l(3)64Cc Kit ELISA, rpd3 Kit ELISA, rpd[3] Kit ELISA, gon-10 Kit ELISA, hdac1b Kit ELISA, rpd3l1 Kit ELISA, Rpd3 Kit ELISA, GB14706 Kit ELISA, hdac1-b Kit ELISA, chunp6919 Kit ELISA, hdac-1 Kit ELISA, mp:zf637-2-001987 Kit ELISA, wu:fb19h11 Kit ELISA, wu:fi06f03 Kit ELISA, zgc:101582 Kit ELISA, zgc:65818 Kit ELISA, Hdac1-ps Kit ELISA, MommeD5 Kit ELISA, ARABIDOPSIS HISTONE DEACETYLASE 1 Kit ELISA, ARABIDOPSIS HISTONE DEACETYLASE 19 Kit ELISA, ATHD1 Kit ELISA, ATHDA19 Kit ELISA, ATRPD3A Kit ELISA, F20D10.250 Kit ELISA, F20D10_250 Kit ELISA, HDA1 Kit ELISA, HDA19 Kit ELISA, HISTONE DEACETYLASE Kit ELISA, HISTONE DEACETYLASE 19 Kit ELISA, HISTONE DEACETYLASE19 Kit ELISA, RPD3A Kit ELISA, histone deacetylase 1 Kit ELISA, HDM Kit ELISA, ab21 Kit ELISA, hdac1a Kit ELISA, histone deacetylase 1 Kit ELISA, Histone deacetylase 1 Kit ELISA, histone deacetylase Kit ELISA, histone deacetylase 1 S homeolog Kit ELISA, histone deacetylase Rpd3 Kit ELISA, histone deacetylase 1 L homeolog Kit ELISA, HDAC1 Kit ELISA, hdac1.S Kit ELISA, LOC411503 Kit ELISA, hdac1 Kit ELISA, Hdac1 Kit ELISA, HD1 Kit ELISA, hda-1 Kit ELISA, hdac1.L Kit ELISA, LOC748850 Kit ELISA

Sujet: Alternative name: HDAC1, GON10, HD1, RPD3, RPD3L1

ID gène: 297893

UniProt: Q4QQW4

Pathways: Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Mitotic G1-G1/S Phases, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Negative Regulation of intrinsic apoptotic Signaling, Embryonic Body Morphogenesis