TNFSF4 Kit ELISA
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- Antigène Voir toutes TNFSF4 Kits ELISA
- TNFSF4 (Tumor Necrosis Factor (Ligand) Superfamily, Member 4 (TNFSF4))
- Épitope
- AA 51-198
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Reactivité
- Souris
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 62.5-4000 pg/mL
- Seuil minimal de détection
- 62.5 pg/mL
- Application
- ELISA
- Fonction
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse TNFSF4/OX40L
- Marque
- PicoKine™
- Type d'échantillon
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- Specificité
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Expression system for standard: NSO
Immunogen sequence: S51-L198 - Réactivité croisée (Details)
- There is no detectable cross-reactivity with other relevant proteins.
- Sensibilité
- <10pg/mL
- Matériel non inclus
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- Immunogène
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Expression system for standard: NSO
Immunogen sequence: S51-L198 - Featured
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- Indications d'application
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- Plaque
- Pre-coated
- Protocole
- mouse TNFSF4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TNFSF4 has been precoated onto 96-well plates. Standards(NSO, S51-L198) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFSF4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TNFSF4 amount of sample captured in plate.
- Procédure de l'essai
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Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse TNFSF4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse TNFSF4 standard solution and each sample be measured in duplicate.
- Précision du teste
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- Sample 1: n=16, Mean(pg/ml): 534, Standard deviation: 23.5, CV(%): 4.4
- Sample 2: n=16, Mean(pg/ml): 1107, Standard deviation: 43.2, CV(%): 3.9
- Sample 3: n=16, Mean(pg/ml): 2374, Standard deviation: 125.8, CV(%): 5.3,
- Sample 1: n=24, Mean(pg/ml): 639, Standard deviation: 37.1, CV(%): 5.8
- Sample 2: n=24, Mean(pg/ml): 1320, Standard deviation: 60.7, CV(%): 4.6
- Sample 3: n=24, Mean(pg/ml): 2448, Standard deviation: 156.7, CV(%): 6.4
- Restrictions
- For Research Use only
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- Conseil sur la manipulation
- Avoid multiple freeze-thaw cycles.
- Stock
- -20 °C,4 °C
- Stockage commentaire
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- Date de péremption
- 12 months
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- Antigène Voir toutes TNFSF4 Kits ELISA
- TNFSF4 (Tumor Necrosis Factor (Ligand) Superfamily, Member 4 (TNFSF4))
- Autre désignation
- TNFSF4 (TNFSF4 Produits)
- Synonymes
- TNFSF4 Kit ELISA, OX40L Kit ELISA, TNFRSF4 Kit ELISA, CD134L Kit ELISA, CD252 Kit ELISA, GP34 Kit ELISA, OX-40L Kit ELISA, OX4OL Kit ELISA, TXGP1 Kit ELISA, Ath-1 Kit ELISA, Ath1 Kit ELISA, Ox40l Kit ELISA, Txgp1l Kit ELISA, gp34 Kit ELISA, TNF superfamily member 4 Kit ELISA, tumor necrosis factor (ligand) superfamily, member 4 Kit ELISA, TNFSF4 Kit ELISA, Tnfsf4 Kit ELISA
- Sujet
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Protein Function: Cytokine that binds to TNFRSF4. Co-stimulates T-cell proliferation and cytokine production.
Background: OX40L is the ligand for CD134 and is expressed on such cells as DC2s enabling amplification of Th2 cell differentiation. OX40L has also been designated CD252. By analysis of an interspecific backcross, Baum et al.(1994) mapped the mouse Tnfsf4 gene to chromosome 1. Using fluorescence in situ hybridization, they localized the human TNFSF4 gene to 1q25, a region sharing homology of synteny with the portion of mouse chromosome 1 containing the Tnfsf4 gene.OX40L strongly inhibited the generation of IL-10-producing Tr1 cells induced by two physiologic stimuli, the inducible costimulatory ligand and immature dendritic cells. In addition, OX40L strongly inhibited IL-10 production and suppressive function of differentiated IL-10-producing Tr1 cells. Tnfsf4 underlies Ath1 in mice and that polymorphisms in its human homolog TNFSF4 increase the risk of myocardial infarction in humans.
Synonyms: Tumor necrosis factor ligand superfamily member 4,OX40 ligand,OX40L,CD252,Tnfsf4,Ox40l, Txgp1l,
Full Gene Name: Tumor necrosis factor ligand superfamily member 4
Cellular Localisation: Membrane, Single-pass type II membrane protein. - ID gène
- 22164
- UniProt
- P43488
- Pathways
- Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation, Cancer Immune Checkpoints
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