TNFSF4 Kit ELISA

N° du produit ABIN6720284

Détail du produit TNFSF4 Kit ELISA

Antigène: TNFSF4 (Tumor Necrosis Factor (Ligand) Superfamily, Member 4 (TNFSF4))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5 pg/mL - 4000 pg/mL

Seuil minimal de détection: 62.5 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: CD 134L, CD 252, CD134, CD134 ligand, CD134L, CD252, CD252 antigen, glycoprotein 34 kd, Glycoprotein Gp34, GP34, OX-40L, OX40 antigen ligand, OX40 ligand, OX40L, TAX transcriptionally-activated glycoprotein 1, TNFL4_HUMAN, Tnfsf4, Tumor necrosis factor (ligand) superfamily member 4, Tumor necrosis factor ligand superfamily member 4, TXGP1

Background: OX40L is the ligand for CD134 and is expressed on such cells as DC2s enabling amplification of Th2 cell differentiation. OX40L has also been designated CD252. By analysis of an interspecific backcross, Baum et al.(1994) mapped the mouse Tnfsf4 gene to chromosome 1. Using fluorescence in situ hybridization, they localized the human TNFSF4 gene to 1q25, a region sharing homology of synteny with the portion of mouse chromosome 1 containing the Tnfsf4 gene.OX40L strongly inhibited the generation of IL-10-producing Tr1 cells induced by two physiologic stimuli, the inducible costimulatory ligand and immature dendritic cells. In addition, OX40L strongly inhibited IL-10 production and suppressive function of differentiated IL-10-producing Tr1 cells. Tnfsf4 underlies Ath1 in mice and that polymorphisms in its human homolog TNFSF4 increase the risk of myocardial infarction in humans.

Gene Name: TNFSF4

Production: Natural and recombinant mouse TNFSF4. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: S51-L198

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse TNFSF4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse TNFSF4 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 62.5pg/mL-4000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: TNFSF4 (Tumor Necrosis Factor (Ligand) Superfamily, Member 4 (TNFSF4))

Autre désignation: TNFSF4 - OX40L (TNFSF4 Produits)

Synonymes: TNFSF4 Kit ELISA, OX40L Kit ELISA, TNFRSF4 Kit ELISA, CD134L Kit ELISA, CD252 Kit ELISA, GP34 Kit ELISA, OX-40L Kit ELISA, OX4OL Kit ELISA, TXGP1 Kit ELISA, Ath-1 Kit ELISA, Ath1 Kit ELISA, Ox40l Kit ELISA, Txgp1l Kit ELISA, gp34 Kit ELISA, TNF superfamily member 4 Kit ELISA, tumor necrosis factor (ligand) superfamily, member 4 Kit ELISA, TNFSF4 Kit ELISA, Tnfsf4 Kit ELISA

ID gène: 22164

NCBI Accession: NP_033478

UniProt: P43488

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation, Cancer Immune Checkpoints