MYD88 Kit ELISA

N° du produit ABIN1672854

Ce kit ELISA Humain MYD88 est un kit ELISA Colorimetric conçu pour quantifier Humain MYD88.

Aperçu rapide pour MYD88 Kit ELISA (ABIN1672854)

Antigène: MYD88 (Myeloid Differentiation Primary Response Gene (88) (MYD88))

Épitope: AA 1-296

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 312-20000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant

Détail du produit MYD88 Kit ELISA

Seuil minimal de détection: 312 pg/mL

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human MyD88

Marque: PicoKine™

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli
Immunogen sequence: M1-P296

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: E.coli
Immunogen sequence: M1-P296

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Contains 1 death domain.

Tissue Specificity: Ubiquitous. .

Plaque: Pre-coated

Protocole: human MyD88 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MyD88 has been precoated onto 96-well plates. Standards(E.coli, M1-P296) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MyD88 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MyD88 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human MyD88 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human MyD88 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(ng/ml): 2.1, Standard deviation: 0.13, CV(%): 6.2
• Sample 2: n=16, Mean(ng/ml): 7, Standard deviation: 0.371, CV(%): 5.3
• Sample 3: n=16, Mean(ng/ml): 13.7, Standard deviation: 0.7, CV(%): 5.1,
• Sample 1: n=24, Mean(ng/ml): 2.7, Standard deviation: 0.189, CV(%): 7
• Sample 2: n=24, Mean(ng/ml): 8.6, Standard deviation: 0.559, CV(%): 6.5
• Sample 3: n=24, Mean(ng/ml): 15.2, Standard deviation: 0.882, CV(%): 5.8

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: MYD88 (Myeloid Differentiation Primary Response Gene (88) (MYD88))

Autre désignation: MYD88

Sujet:

Protein Function: Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway. Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes. MyD88-mediated signaling in intestinal epithelial cells is crucial for maintenance of gut homeostasis and controls the expression of the antimicrobial lectin REG3G in the small intestine. .

Background: Myeloid differentiation primary response gene(88)(MYD88) is a protein that, in humans, is encoded by the MYD88 gene. By interspecific backcross mapping, the mouse MyD88 gene was localized to chromosome 9, the human homolog was mapped to 3p22-p21.3 The human ortholog MYD88 seems to function similarly to mice, however, available evidence suggests that MYD88 is dispensable for human resistance to common viral infections and to all but a few pyogenic bacterial infections, demonstrating a major difference between mouse and human immune responses.

Synonyms: Myeloid differentiation primary response protein MyD88,MYD88,

Full Gene Name: Myeloid differentiation primary response protein MyD88

Cellular Localisation: Cytoplasm.

ID gène: 4615

UniProt: Q99836

Pathways: Signalisation NF-kappaB, Signalisation TLR, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades