SensoLyte® Calcein Cell Viability Assay Kit

N° du produit ABIN1882422

Détail du produit

Antigène: Calcein AM

Méthode de détection: Fluorometric

Application: Enzyme Activity Assay (EAA)

Marque: SensoLyte®

Attributs du produit: The SensoLyte® Calcein Cell Viability Assay Kit provides a convenient tool to detect live cells. This kit can be used in proliferation, cytotoxicity, invasion, adhesion, migration and other cell-based assays.

Information d'application

Commentaires:

Enzymatic Activity Assay kit

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Protect Component A from light and moisture.

Stock: -20 °C

Stockage commentaire: Store Component A at -20 °C. Component B can be stored at room temperature for convenience.

Référence

Saha, Takahashi, Kurebayashi, Fukushima, Minami, Kinbara, Ichitani, Sagesaka, Suzuki: "Antiviral effect of strictinin on influenza virus replication." dans: Antiviral research, Vol. 88, Issue 1, pp. 10-8, (2010) (PubMed).

Détail du antigène

Antigène: Calcein AM

Sujet: Calcein AM (calcein acetoxymethyl ester) is a non-fluorescent reagent that easily permeates cellular membrane. Once it permeates live cells, esterases remove the acetomethoxy group allowing calcein to bind to calcium, which results in a strong green fluorescence. The increase of fluorescence is proportional to the number of live cells. Inside dead cells, the non-fluorescent calcein AM remains intact due to the lack of active esterases. The fluorescent signal from the conversion of calcein AM to calcein can be monitored at Ex/Em=494nm /520 nm.