SensoLyte® 520 Cathepsin B Assay Kit

N° du produit ABIN1882436

Détail du produit

Antigène: Cathepsin B (CTSB)

Méthode de détection: Fluorometric

Application: Fluorescence Resonance Energy Transfer Microscopy (FRET)

Marque: SensoLyte®

Attributs du produit: The SensoLyte® 520 Cathepsin B Activity Assay Kit provides a QXL™ 520/HiLyte FluorTM 488 labeled FRET peptide substrate for measurement of enzyme activity. In the intact FRET peptide, the fluorescence of HiLyte FluorTM 488 is quenched by QXL™ 520. Upon cleavage of the FRET peptide by the active enzyme, the increase of fluorescence can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, the QXL™ 520/HiLyte FluorTM 488 based FRET peptide has less interference from the autofluorescence of test compounds and cellular components and provides better assay sensitivity.

Information d'application

Commentaires:

FRET-based Assay Kit

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Protect Components A and B from light and moisture.

Stock: -80 °C

Stockage commentaire: Store all kit components at -20 °C, except for Component C. Store Component C at -80 °C. Component D can be stored at room temperature for convenience

Référence

Unno, Wakamori, Koike, Uchiyama, Ishikawa, Kubota, Yoshida, Sasakawa, Peters, Mizusawa, Watase: "Development of Purkinje cell degeneration in a knockin mouse model reveals lysosomal involvement in the pathogenesis of SCA6." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 43, pp. 17693-8, (2012) (PubMed).

Détail du antigène

Antigène: Cathepsin B (CTSB)

Autre désignation: Cathepsin B (CTSB Produits)

Synonymes: APPS Kit, CPSB Kit, CB Kit, apps Kit, cpsb Kit, LOC692390 Kit, CTSB Kit, cathepsin B Kit, cathepsin B S homeolog Kit, CTSB Kit, Ctsb Kit, ctsb.S Kit, LOC692390 Kit

Sujet: Cathepsins are a class of globular lysosomal proteases, playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin B is a member of the cathepsin family consisting of 12 cysteine proteases with broad exo- and endopeptidase activity. Elevated levels of cathepsin B were detected in metastases and neurological disorders including Alzheimer's disease (AD).