SensoLyte® 390 Generic MMP Activity Kit

N° du produit ABIN1882504

Détail du produit

Antigène: Matrix Metalloproteinase

Méthode de détection: Fluorometric

Application: Fluorescence Resonance Energy Transfer Microscopy (FRET), Activation (Act)

Marque: SensoLyte®

Attributs du produit: The SensoLyte® 390 Generic MMP Assay Kit is optimized to detect the activity of a variety of MMPs, including MMP-1, 2, 7, 8, 9, 13, 14, 15, 16, and 24. This kit can be used for detecting generic MMP activity in biological samples or for high throughput screening of MMP inducers and inhibitors using purified MMPs. This kit provides an Mca/Dnp fluorescence resonance energy transfer (FRET) peptide as a MMP substrate. In the intact FRET peptide, the fluorescence of Mca is quenched by Dnp. Upon cleavage into two separate fragments by MMPs, the fluorescence of Mca is recovered, and can be monitored at excitation/emission wavelengths = 330 nm/390 nm. The assays are performed in a convenient 96-well microplate format.

Information d'application

Commentaires:

FRET-based Assay Kit

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Protect Components A and B from light.

Stock: -20 °C

Stockage commentaire: Store all components at -20 °C. Components D and E can be stored at 4 °C for convenience.

Référence

Zeng, Adamson, Curry, Tarbell: "Sphingosine-1-phosphate protects endothelial glycocalyx by inhibiting syndecan-1 shedding." dans: American journal of physiology. Heart and circulatory physiology, Vol. 306, Issue 3, pp. H363-72, (2014) (PubMed).

Détail du antigène

Antigène: Matrix Metalloproteinase

Autre désignation: MMP

Sujet: Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components. MMPs play important roles in tumor development and invasion, as well in many diseases.