IL1R1 Kit ELISA

N° du produit ABIN1889296

Détail du produit IL1R1 Kit ELISA

Antigène: IL1R1 (Interleukin 1 Receptor, Type I (IL1R1))

Épitope: AA 21-332

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 125-8000 pg/mL

Seuil minimal de détection: 125 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL1R1

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: D21-T332

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: D21-T332

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Belongs to the interleukin-1 receptor family.

Plaque: Pre-coated

Protocole: human IL1R1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL1R1 has been precoated onto 96-well plates. Standards(NSO, D21-T332) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL1R1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL1R1 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 8000pg/mL, 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL human IL1R1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IL1R1 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 746, Standard deviation: 30.59, CV(%): 4.1
• Sample 2: n=16, Mean(pg/ml): 3374, Standard deviation: 175.5, CV(%): 5.2
• Sample 3: n=16, Mean(pg/ml): 5587, Standard deviation: 312.9, CV(%): 5.6,
• Sample 1: n=24, Mean(pg/ml): 857, Standard deviation: 59.13, CV(%): 6.9
• Sample 2: n=24, Mean(pg/ml): 3345, Standard deviation: 250.9, CV(%): 7.5
• Sample 3: n=24, Mean(pg/ml): 6558, Standard deviation: 518.1, CV(%): 7.9

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: IL1R1 (Interleukin 1 Receptor, Type I (IL1R1))

Autre désignation: IL1R1 (IL1R1 Produits)

Synonymes: CD121A Kit ELISA, D2S1473 Kit ELISA, IL-1R-alpha Kit ELISA, IL1R Kit ELISA, IL1RA Kit ELISA, P80 Kit ELISA, CD121a Kit ELISA, CD121b Kit ELISA, IL-iR Kit ELISA, Il1r-1 Kit ELISA, DER4 Kit ELISA, Fit-1 Kit ELISA, Ly84 Kit ELISA, ST2L Kit ELISA, St2 Kit ELISA, St2-rs1 Kit ELISA, T1 Kit ELISA, T1/ST2 Kit ELISA, interleukin 1 receptor type 1 Kit ELISA, interleukin 1 receptor, type I Kit ELISA, interleukin 1 receptor-like 1 Kit ELISA, IL1R1 Kit ELISA, Il1r1 Kit ELISA, Il1rl1 Kit ELISA

Sujet:

Protein Function: Receptor for IL1A, IL1B and IL1RN. After binding to interleukin-1 associates with the corecptor IL1RAP to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B, MAPK and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Binds ligands with comparable affinity and binding of antagonist IL1RN prevents association with IL1RAP to form a signaling complex. .

Background: Interleukin 1 receptor, type I(IL1R1), also known as CD121a(Cluster of Differentiation 121a), is an interleukin receptor. The protein encoded by this gene is a cytokine receptor that belongs to the interleukin-1 receptor family. IL1R1 along with interleukin 1 receptor, type II(IL1R2), interleukin 1 receptor-like 2(IL1RL2), and interleukin 1 receptor-like 1(IL1RL1) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12. This protein is a receptor for interleukin 1 alpha(IL1A), interleukin 1 beta(IL1B), and interleukin 1 receptor antagonist(IL1RA). It is an important mediator involved in many cytokine induced immune and inflammatory responses.

Synonyms: Interleukin-1 receptor type 1,IL-1R-1,IL-1RT-1,IL-1RT1,CD121 antigen-like family member A,Interleukin-1 receptor alpha,IL-1R-alpha,Interleukin-1 receptor type I,p80,CD121a,Interleukin-1 receptor type 1, membrane form,mIL-1R1,mIL-1RI,Interleukin-1 receptor type 1, soluble form,sIL-1R1,sIL-1RI,IL1R1,IL1R, IL1RA, IL1RT1,

Full Gene Name: Interleukin-1 receptor type 1

Cellular Localisation: Membrane, Single-pass type I membrane protein . Cell membrane . Secreted.

ID gène: 3554

UniProt: P14778

Pathways: Signalisation NF-kappaB, Carbohydrate Homeostasis, Cancer Immune Checkpoints