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EGFR Kit ELISA

EGFR Reactivité: Humain phosphorylated Colorimetric Cell ELISA Cell Culture Cells
N° du produit ABIN1981821
  • Antigène Voir toutes EGFR Kits ELISA
    EGFR (Epidermal Growth Factor Receptor (EGFR))
    Épitope
    phosphorylated
    Reactivité
    • 34
    • 25
    • 23
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Cell ELISA
    Application
    ELISA
    Fonction
    Cell-Based Human EGFR (Activated) Phosphorylation ELISA Kit. Suitable for adherent whole cell lines.
    Marque
    CellBIND®,RayBio®
    Type d'échantillon
    Cell Culture Cells
    Analytical Method
    Semi-Quantitative
    Specificité
    The antibodies provided in this kit recognizes human Tyrosine-phosphorylated-EGFR and total EGFR for comparison.
    Attributs du produit
    • Site and signal pathway-specific
    • In vitro detection of adherent cell culture
    • No sample lysis needed
    • Compatible with a standard ELISA plate reader
    • Faster results than with ELISA
    • Adaptable for high-throughput screening and drug discovery
    Ingrédients
    • uncoated 96-well Microplate
    • Wash Buffer A
    • Wash Buffer B
    • Fixing Solution
    • Quenching Buffer
    • Blocking Buffer
    • Anti-phospho antibody
    • Anti-pan antibody
    • HRP-Conjugated Secondary Antibody
    • TMB One-Step Substrate
    • Stop Solution
    Matériel non inclus
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Featured
    Discover our best selling EGFR Kit ELISA
    Top Product
    Discover our top product EGFR Kit ELISA
  • Volume d'échantillon
    100 μL
    Plaque
    Uncoated
    Protocole
    1. Seed 10,000-30,000 cells into each well and incubate overnight.
    2. Apply various treatment, inhibitors or activators according to manufacture's instructions.
    3. Add 100 μL of Fixing Solution into each well and incubate for 20 min at RT with shaking.
    4. Add 200 μL of prepared 1X Quenching Buffer and incubate 20 min at RT.
    5. Add 200 μL of Blocking Solution and incubate for 1 h at 37 °C.
    6. Add 50 μL of 1X anti-phospho-protein specific antibody or anti-pan-protein specific antibody to each well and incubate for 2 h at RT.
    7. Add 50 μL of prepared 1X HRP-Anti-Rabbit or Mouse IgG and incubate for 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Préparation des réactifs

    NOTE: Thaw all reagents to room temperature immediately before use. If wash buffers contain visible crystals, warm to room temperature and mix gently until dissolved.
    NOTE: Briefly centrifuge (~1,000g) ITEMS G, H, and I before opening to ensure maximum recovery.

    Item, Component, Preparation, Example
    A, Uncoated 96 Well Microplate, No Preparation, N/A
    B, 20x Wash Buffer A concentrate, Dilute 20-fold with destilled or deionized water, 25 ml of concentrate +475 ml of water = 500 ml of 1x working solution
    C, 20x Wash Buffer B concentrate, Dilute 20-fold with destilled or deionized water, 25 ml of concentrate +475 ml of water = 500 ml of 1x working solution
    D, Fixing Solution, No Preparation, N/A
    E, 30X Quenching Buffer Concentrate, Dilute 30-fold with 1X Wash Buffer A, 1 ml of concentrate + 29 ml of wash buffer = 30 ml of 1X working solution
    F, 5X Blocking Buffer Concentrate, Dilute 5-fold with distilled or deionized water, 20 ml of concentrate + 80 ml of water = 100 ml of 1X working solution
    Primary Antibodies:
    G, 1000x Mouse Anti-phospho (Activated) EGFR Concentrate , Dilute 1000-fold with 1x Blocking Buffer, 2 µL of concentrate + 1998 µL of 1x Blocking Buffer = 2 ml of 1x working solution
    H, 1000x Mouse anti-EGFR Concentrate, Dilute 1000-fold with 1x Blocking Buffer, 2 µL of concentrate + 1998 µL of 1x Blocking Buffer = 2 ml of 1x working solution
    Secondary Antibody:
    I, 1000x HRP Conjugated Anti-Mouse IgG Concentrate, Dilute 1000-fold with 1x Blocking Buffer, 5 µL of concentrate + 4995 µL of 1x Blocking Buffer = 5 ml of 1x working solution

    J, TMB Substrate, No Preparation, N/A
    K, Stop Solution, No Preparation, N/A

    Procédure de l'essai

    NOTE: ALL incubations and wash steps must be performed under gentle rocking or rotation (~1-2 cycles/sec).
    1. Design your experiment. For example, see in Figure 2 below.
    OPTIONAL: If seeding HUVECs, HMEC-1 or other loosely attached cells, coat the Uncoated 96-Well Microplate (ITEM A) by adding 100 µL poly-L-Lysine (Recommended Sigma Aldrich) into each well and then follow manufacturer's instructions. A pre-coated CellBIND® microplate or other poly-lysine treated tissue culture plate may be used in place of Item A.
    2. Seed 100 µL of 30,000 cells into each well of the Uncoated 96-Well Microplate (ITEM A) provided and incubate overnight at 37 °C with 5 % CO2.
    NOTE: The optimal cell number used will vary on the cell line and the relative amount of protein phosphorylation. More or less cells may be used but this must be determined empirically.
    NOTE: The cells can be starved ~4-24 hours (depending on cell line) prior to treatment with inhibitors or activators.
    3. Apply various treatments, inhibitors (such as siRNA or chemicals) or activators according to manufacturer's instructions and incubate for the desired time points.
    NOTE: It is recommended to dissolve inhibitors or activators into serum-free cell culture medium before treating the cells (unless otherwise stated in the manufacturer's instructions.)
    4. Discard the cell culture medium by flipping the microplate upside down and gently tapping the bottom of the microplate over a sink.
    5. Wash by pipetting 200 µL of the prepared 1X Wash Buffer A (ITEM B) into each well. Discard the wash buffer (same as step 4) and wash 2 more times for a total of 3 washes using fresh wash buffer each time. After the final wash, gently blot the microplate onto a paper towel to remove any excess/remaining buffer.
    NOTE: To avoid cell loss, do not pipette directly onto the cells. Instead, gently dispense the liquid down the wall of cell culture wells. Also avoid the use of vacuum suction or too forcefully tapping the microplate when discarding any solution.
    6. Add 100 µL of Fixing Solution (ITEM D) into each well and incubate for 20 minutes at room temperature.
    NOTE: The fixing solution is used to permeabilize the cells.
    7. Repeat wash step 5.
    8. Add 200 µL of the prepared 1X Quenching Buffer (ITEM E) into each well and incubate 20 minutes at room temperature.
    NOTE: The quenching buffer is used to minimize the background response.
    9. Wash 4 times with 1X Wash Buffer A.
    10. Add 200 µL of the prepared 1X Blocking Buffer (ITEM F) into each well and incubate for 1 hour at 37 °C.
    11. Wash 3 times with the prepared 1X Wash Buffer B (ITEM C).
    NOTE: If needed, the microplate may be stored at -80 °C for several days after this wash.
    12. Add 50 µL of the prepared 1X primary antibody (ITEM G or H) into each corresponding well and incubate for 2 hours at room temperature.
    13. Wash 4 times with 1X Wash Buffer B.
    14. Add 50 µL of 1X HRP Conjugated secondary antibody (ITEM I) into each well and incubate for 1 hour at room temperature.
    15. Wash 4 times with 1X Wash Buffer B.
    16. Add 100 µL of the TMB Substrate (ITEM J) into each well and incubate for 30 minutes at room temperature in the dark.
    17. Add 50 µL of the Stop Solution (ITEM K) into each well. Read at 450 nm immediately.

    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.
    Date de péremption
    6 months
  • Boonanantanasarn, Gill, Yap, Jayaprakash, Sullivan, Gill: "Enterococcus faecalis enhances cell proliferation through hydrogen peroxide-mediated epidermal growth factor receptor activation." dans: Infection and immunity, Vol. 80, Issue 10, pp. 3545-58, (2012) (PubMed).

    Joyner, Jones, Lessnick, Schiffman, Randall: "Potential for modulation of the fas apoptotic pathway by epidermal growth factor in sarcomas." dans: Sarcoma, Vol. 2011, pp. 847409, (2012) (PubMed).

    Wang, Patil, Li, Humphrey, Brattain, Howell: "Activation of the TGFalpha autocrine loop is downstream of IGF-I receptor activation during mitogenesis in growth factor dependent human colon carcinoma cells." dans: Oncogene, Vol. 21, Issue 18, pp. 2785-96, (2002) (PubMed).

    Hirota, Murata, Itoh, Yodoi, Fukuda: "Redox-sensitive transactivation of epidermal growth factor receptor by tumor necrosis factor confers the NF-kappa B activation." dans: The Journal of biological chemistry, Vol. 276, Issue 28, pp. 25953-8, (2001) (PubMed).

  • Antigène Voir toutes EGFR Kits ELISA
    EGFR (Epidermal Growth Factor Receptor (EGFR))
    Autre désignation
    EGFR (EGFR Produits)
    Synonymes
    C-erb Kit ELISA, CG10079 Kit ELISA, D-EGFR Kit ELISA, D-Egf Kit ELISA, DEGFR Kit ELISA, DER Kit ELISA, DER flb Kit ELISA, DER/EGFR Kit ELISA, DER/faint little ball Kit ELISA, DER/top Kit ELISA, DER/torpedo Kit ELISA, DER1 Kit ELISA, DEgfr Kit ELISA, Degfr Kit ELISA, Der Kit ELISA, DmHD-33 Kit ELISA, Dmel\\CG10079 Kit ELISA, EFG-R Kit ELISA, EGF-R Kit ELISA, EGFR Kit ELISA, EGFr Kit ELISA, EGfr Kit ELISA, EK2-6 Kit ELISA, Egf Kit ELISA, Egf-r Kit ELISA, EgfR Kit ELISA, El Kit ELISA, Elp Kit ELISA, Elp-1 Kit ELISA, Elp-B1 Kit ELISA, Elp-B1RB1 Kit ELISA, HD-33 Kit ELISA, TOP Kit ELISA, Torpedo/DER Kit ELISA, Torpedo/Egfr Kit ELISA, c-erbB Kit ELISA, d-egf-r Kit ELISA, dEGFR Kit ELISA, dEGFR1 Kit ELISA, dEgfr Kit ELISA, der Kit ELISA, egfr Kit ELISA, flb Kit ELISA, l(2)05351 Kit ELISA, l(2)09261 Kit ELISA, l(2)57DEFa Kit ELISA, l(2)57EFa Kit ELISA, l(2)57Ea Kit ELISA, mor1 Kit ELISA, top Kit ELISA, top/DER Kit ELISA, top/flb Kit ELISA, torpedo/Egfr Kit ELISA, torpedo/egfr Kit ELISA, EGFR12 Kit ELISA, EGFR15 Kit ELISA, egfr1 Kit ELISA, Erbb2 Kit ELISA, ERBB Kit ELISA, ERBB1 Kit ELISA, HER1 Kit ELISA, PIG61 Kit ELISA, mENA Kit ELISA, ErbB-1 Kit ELISA, Errp Kit ELISA, 9030024J15Rik Kit ELISA, AI552599 Kit ELISA, Erbb Kit ELISA, Errb1 Kit ELISA, Wa5 Kit ELISA, wa-2 Kit ELISA, wa2 Kit ELISA, epidermal growth factor receptor Kit ELISA, Epidermal growth factor receptor Kit ELISA, epidermal growth factor receptor a (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian) Kit ELISA, EGFR Kit ELISA, Egfr Kit ELISA, egfra Kit ELISA, egfr1 Kit ELISA, LOC5564544 Kit ELISA
    ID gène
    1956
    UniProt
    P00533
    Pathways
    Signalisation NF-kappaB, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins
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