HSD11B2 Kit ELISA

N° du produit ABIN2042729

Détail du produit HSD11B2 Kit ELISA

Antigène: HSD11B2 (Hydroxysteroid (11-Beta) Dehydrogenase 2 (HSD11B2))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.78-50 ng/mL

Seuil minimal de détection: 0.78 ng/mL

Application: ELISA

Marque: BioAssay™

Sensibilité: < 0.27 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: HSD11B2 (Hydroxysteroid (11-Beta) Dehydrogenase 2 (HSD11B2))

Autre désignation: 11-beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2) (HSD11B2 Produits)

Synonymes: MGC81883 Kit ELISA, 11-HSD2 Kit ELISA, AME Kit ELISA, AME1 Kit ELISA, HSD11K Kit ELISA, HSD2 Kit ELISA, SDR9C3 Kit ELISA, 11HSD2 Kit ELISA, HSD11B2 Kit ELISA, hydroxysteroid (11-beta) dehydrogenase 2 L homeolog Kit ELISA, hydroxysteroid 11-beta dehydrogenase 2 Kit ELISA, hsd11b2.L Kit ELISA, HSD11B2 Kit ELISA, Hsd11b2 Kit ELISA

Pathways: Steroid Hormone Biosynthesis, Regulation of Systemic Arterial Blood Pressure by Hormones