CHEK1 Kit ELISA

N° du produit ABIN2043125

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Souris CHEK1.

Aperçu rapide pour CHEK1 Kit ELISA (ABIN2043125)

Antigène: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.15-10 ng/mL

Application: ELISA

Détail du produit CHEK1 Kit ELISA

Seuil minimal de détection: 0.15 ng/mL

Sensibilité: < 0.058 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Checkpoint Homolog (CHEK1) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Checkpoint Homolog (CHEK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Checkpoint Homolog (CHEK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Checkpoint Homolog (CHEK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Checkpoint Homolog (CHEK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Autre désignation: Checkpoint Homolog (CHEK1)

Pathways: Signalisation p53, Apoptose, Cycle Cellulaire, Réparation de l'ADN