CYP27A1 Kit ELISA

N° du produit ABIN2043271

Détail du produit CYP27A1 Kit ELISA

Antigène: CYP27A1 (Cytochrome P450, Family 27, Subfamily A, Polypeptide 1 (CYP27A1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.15-10 ng/mL

Seuil minimal de détection: 0.15 ng/mL

Application: ELISA

Marque: BioAssay™

Sensibilité: < 0.057 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Cytochrome P450 27A1 (CYP27A1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 27A1 (CYP27A1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 27A1 (CYP27A1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 27A1 (CYP27A1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Cytochrome P450 27A1 (CYP27A1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: CYP27A1 (Cytochrome P450, Family 27, Subfamily A, Polypeptide 1 (CYP27A1))

Autre désignation: Cytochrome P450 27A1 (CYP27A1) (CYP27A1 Produits)

Synonymes: CP27 Kit ELISA, CTX Kit ELISA, CYP27 Kit ELISA, Cyp27 Kit ELISA, P450C27 Kit ELISA, 1300013A03Rik Kit ELISA, cytochrome P450 family 27 subfamily A member 1 Kit ELISA, cytochrome P450, family 27, subfamily a, polypeptide 1 Kit ELISA, cytochrome P450, family 27, subfamily A, polypeptide 1 Kit ELISA, sterol 26-hydroxylase, mitochondrial Kit ELISA, CYP27A1 Kit ELISA, Cyp27a1 Kit ELISA, cyp27a1 Kit ELISA