FAH Kit ELISA

N° du produit ABIN2043517

Détail du produit FAH Kit ELISA

Antigène: FAH (Fumarylacetoacetate Hydrolase (Fumarylacetoacetase) (FAH))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.15-10 ng/mL

Seuil minimal de détection: 0.15 ng/mL

Application: ELISA

Marque: BioAssay™

Sensibilité: < 0.055 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Fumarylacetoacetate Hydrolase (FAH) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fumarylacetoacetate Hydrolase (FAH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fumarylacetoacetate Hydrolase (FAH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fumarylacetoacetate Hydrolase (FAH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Fumarylacetoacetate Hydrolase (FAH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: FAH (Fumarylacetoacetate Hydrolase (Fumarylacetoacetase) (FAH))

Autre désignation: Fumarylacetoacetate Hydrolase (FAH) (FAH Produits)

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