HAase Kit ELISA

N° du produit ABIN2043721

Détail du produit HAase Kit ELISA

Antigène: HAase (Hyaluronidase (HAase))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 78.12-5000 pg/mL

Seuil minimal de détection: 78.12 pg/mL

Application: ELISA

Marque: BioAssay™

Sensibilité: < 36 pg/mL

Information d'application

Plaque: Pre-coated

Protocole: Hyaluronidase (HAase) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hyaluronidase (HAase). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hyaluronidase (HAase). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hyaluronidase (HAase), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Hyaluronidase (HAase) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: HAase (Hyaluronidase (HAase))

Abstract: HAase Produits

Synonymes: hyaluronidase Kit ELISA, Hyaluronidase Kit ELISA, hylP Kit ELISA, chhy-1 Kit ELISA, hylP.1 Kit ELISA, hylP.4 Kit ELISA

Pathways: Glycosaminoglycan Metabolic Process