Sema4a Kit ELISA

N° du produit ABIN2044517

Détail du produit Sema4a Kit ELISA

Antigène: Sema4a (Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4A (Sema4a))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.31-20 ng/mL

Seuil minimal de détection: 0.31 ng/mL

Application: ELISA

Marque: BioAssay™

Sensibilité: < 0.131 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Semaphorin 4A (SEMA4A) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Semaphorin 4A (SEMA4A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Semaphorin 4A (SEMA4A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Semaphorin 4A (SEMA4A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Semaphorin 4A (SEMA4A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: Sema4a (Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4A (Sema4a))

Autre désignation: Semaphorin 4A (SEMA4A) (Sema4a Produits)

Synonymes: SEMA4A Kit ELISA, CORD10 Kit ELISA, RP35 Kit ELISA, SEMAB Kit ELISA, SEMB Kit ELISA, AI132332 Kit ELISA, SemB Kit ELISA, Semab Kit ELISA, semaphorin 4A Kit ELISA, sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A Kit ELISA, SEMA4A Kit ELISA, sema4a Kit ELISA, Sema4a Kit ELISA