TNF alpha Kit ELISA

N° du produit ABIN2112010

Kit ELISA Souris TNF alpha, test Colorimetric pour la quantification de Souris TNF alpha.

Aperçu rapide pour TNF alpha Kit ELISA (ABIN2112010)

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6-1000 pg/mL

Application: ELISA

Détail du produit TNF alpha Kit ELISA

Seuil minimal de détection: 15.6 pg/mL

Analytical Method: Quantitative

Sensibilité: < 4.5 pg/mL

Ingrédients: MOUSE TNf-a MICROTITER PLATE
BIOTIN CONJUGATE
AVIDIN CONJUGATE
MOUSE TNF-a STANDARD
CALIBRATOR DILUENT I
CALIBRATOR DILUENT II
WASH BUFFER (20X/96 wells, 30X/192 wells)
SUBSTRATE A
SUBSTRATE B
STOP SOLUTION

Information d'application

Plaque: Pre-coated

Protocole: This mouse TNF-a enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to mouse TNF-a. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated monoclonal antibody preparation specific for mouse TNF-a and incubated. Mouse TNF-a if present, will bind and become immobilized by the antibody pre-coated on the wells and then be -sandwiched- by biotin conjugate. The microtiter plate wells are thoroughly washed to remove unbound mouse TNF-a and other components of the sample. In order to quantify the amount of mouse TNF-a present in the sample, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Avidin is a tetramer containing four identical subunits that each has a high affinity-binding site for biotin. The wells are thoroughly washed to remove all unbound HRP-conjugated Avidin and a TMB (3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain mouse TNF-a, biotin-conjugated antibody, and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Autre désignation: TNF-A

Sujet: TNF-a plays an important role in the immune response to bacterial, and certain fungal, viral, parasitic invasions, in tissue remodeling, autoimmune-diseases and the necrosis of specific tumors. The pleiotropic effect of TNF-a regulation is attributed to its ability to trigger multiple signaling pathways simultaneously. TNF-a hyper-expression in response to the components of some bacteria such as LPS can cause life threatening septic shock. Recombinant TNF-a, in combination with chemotherapy, has been applied for synergistic treatment of soft sarcomas, melanomas and other irresectable tumors. Anti-TNF-a therapy has been used for treatment of rheumatoid arthritis.

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome