SERPINA1 Kit ELISA

N° du produit ABIN2648470

Détail du produit SERPINA1 Kit ELISA

Antigène: SERPINA1 (serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1))

Reactivité: Chemical

Méthode de détection: Colorimetric

Application: ELISA

Fonction: Alpha-1 Antitrypsin ELISA Kit allows an easy, rapid and precise quantitative determination of alpha-1-antitrypsin in biological samples.

Type d'échantillon: Fecal, Serum, Plasma

Analytical Method: Quantitative

Sensibilité: 1.5 ng/ml

Attributs du produit: Alpha-1-Antitrypsin is a 52 kD glycoprotein, which is produced by the liver, intestinal macrophages, monocytes and mucous membrane cells of the gut. It belongs to the group of acute phase proteins and is one of the most important proteinase inhibitors. Alpha-1-antitrypsin inhibits, beside others, the proteinases trypsin and the elastase of neutrophils. A lack of α-1-AT leads to an enhanced proteolysis. Only a very small amount of alpha-1-antitrypsin is cleaved or resorbed in the gut. Therefore the measurement of α-1-AT in stool reflects the permeability of the gut during inflammatory processes. Alpha-1 Antitrypsin ELISA Kit allows an easy, rapid and precise quantitative determination of alpha-1-antitrypsin in biological samples. The kit includes all reagents ready to use for preparation of the samples.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 100 μL

Durée du test: 2 h

Protocole: The Alpha-1 Antitrypsin ELISA assay determines human alpha-1-antitrypsin according to the "sandwich"-principle. Alpha-1 Antitrypsin in sample, standard and controls binds to antibodies, which are coated to the microtiter plate. After a washing step a peroxidase labeled detection antibody is added. A second washing step is followed by the addition of the substrate which is converted to a colored product by the peroxidase. The reaction is terminated by the addition of an acidic stop solution. The optical densities are read at 450 nm (against the reference wavelength 620 nm) in a microtiter plate reader. The α-1-AT concentration can be calculated from the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Détail du antigène

Antigène: SERPINA1 (serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1))

Autre désignation: alpha 1 Antitrypsin (SERPINA1 Produits)

Synonymes: AACT Kit ELISA, ACT Kit ELISA, GIG25 Kit ELISA, A1AT Kit ELISA, PI Kit ELISA, alpha-1-PI Kit ELISA, A1A Kit ELISA, AAT Kit ELISA, PI1 Kit ELISA, PRO2275 Kit ELISA, alpha1AT Kit ELISA, Pi Kit ELISA, Spi1 Kit ELISA, MGC64429 Kit ELISA, serpina1d Kit ELISA, Aps Kit ELISA, SERPINA3-5 Kit ELISA, serpin family A member 3 Kit ELISA, alpha-1-antiproteinase F precursor Kit ELISA, serpin family A member 1 Kit ELISA, alpha-1-antitrypsin Kit ELISA, calumenin Kit ELISA, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 S homeolog Kit ELISA, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 Kit ELISA, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 5 Kit ELISA, SH2B adaptor protein 2 Kit ELISA, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3 Kit ELISA, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 L homeolog Kit ELISA, SERPINA3 Kit ELISA, LOC100008973 Kit ELISA, SERPINA1 Kit ELISA, LOC100328621 Kit ELISA, CALU Kit ELISA, Serpina1 Kit ELISA, serpina1.S Kit ELISA, serpina1 Kit ELISA, SPIA5 Kit ELISA, Sh2b2 Kit ELISA, Calu Kit ELISA, serpina1.L Kit ELISA