Angiotensin II is a peptide hormone which causes vasoconstriction, increased blood pressure, and release of aldosterone from the adrenal cortex. It occupies an important role in the Renin-Angiotensin-System (RAS). Angiotensin II mediates the effects through G-Protein-coupled receptors, the Angiotensin receptors. The occurrence of autoantibodies against AT1 receptor is associated with an increased risk of an immunologic rejection after organ transplantation. The presence of AT1 autoantibodies correlate with the existence and course of Scleroderma. This Rat Anti Angiotensin Receptor 1 IgM Antibody ELISA Assay is designed for the determination of Rat antibodies (IgM) against the Angiotensin II receptor subtype I in serum and plasma.
Optimal working dilution should be determined by the investigator.
Volume d'échantillon
100 μL
Durée du test
3.5 h
Protocole
The Rat Anti Angiotensin Receptor 1 IgM Antibody is an antibody screening test. Angiotensin II Receptor has been pre-coated onto a microtiter plate. During the first incubation the rat anti-Angiotensin II receptor 1-IgM-Antibodies of the samples are immobilized on the plate. The autoantibodies are detected with a POD labelled anti-Rat IgM antibody. In the following enzymatic substrate reaction the intensity of the colour correlates with the concentration and/ or avidity of anti-Angiotensin II receptor 1-antibody (IgM).