Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) concentrations in cell culture supernates, serum, and plasma.
Type d'échantillon
Serum, Plasma, Cell Culture Cells
Analytical Method
Quantitative
Sensibilité
7 pg/ml
Attributs du produit
Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.
Optimal working dilution should be determined by the investigator.
Volume d'échantillon
100 μL
Durée du test
3 h
Plaque
Pre-coated
Protocole
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for GM-CSF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GM-CSF present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for GM-CSF is added to the wells and binds to the combination of capture antibody- GM-CSF in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of GM-CSF present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven GM-CSF standard dilutions and GM-CSF sample concentration determined.