CCL2 Kit ELISA

N° du produit ABIN2690571

Ce kit ELISA Humain CCL2 est un kit ELISA Colorimetric conçu pour quantifier Humain CCL2. Ce Kit ELISA a été cité dans 3+ publications.

Aperçu rapide pour CCL2 Kit ELISA (ABIN2690571)

Antigène: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Type d'échantillon: Serum, Plasma, Cell Culture Supernatant

Détail du produit CCL2 Kit ELISA

Fonction: The Human MCP-1 ELISA Kit is for the quantitative determination of human MCP-1 in serum, plasma, and cell culture supernatant.

Analytical Method: Quantitative

Ingrédients: Antibody Coated Wells - 2 plates of 96 breakable wells (12 strips x 8 wells per plate)
Detection Antibody - 30 mL
Standards - 4 vials, lyophilized
Enzyme Concentrate (250x) - 150 μL
Standard/Sample Diluent - 30 mL
ELISA Diluent - 12 mL
Wash Concentrate (20x) - 100 mL
TMB One-Step Substrate Reagent - 30 mL
Stop Solution - 13 mL
Plate Sealers - 4 each

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

BD OptEIA™ Human MCP-1 ELISA Kit

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: 1. Store unopened kit at 2 - 8°C. Do not use kit after expiration date.
2. Before use, bring all reagents to room temperature (18 - 25°C). Immediately after use, return to proper storage conditions.
3. Lyophilized standards are stable until kit expiration date. After reconstitution, use freshly reconstituted standard within 12 hours (stored at 2 - 8°C).

Références pour CCL2 Kit ELISA (ABIN2690571)

Rollins: "Chemokines." dans: Blood, Vol. 90, Issue 3, pp. 909-28, (1997) (PubMed).

Rollins, Stier, Ernst, Wong: "The human homolog of the JE gene encodes a monocyte secretory protein." dans: Molecular and cellular biology, Vol. 9, Issue 11, pp. 4687-95, (1990) (PubMed).

Capsoni, Minonzio, Ongari, Zanussi: "A new simplified single-filter assay for 'in vitro' evaluation of chemotaxis of 51Cr-labeled polymorphonuclear leukocytes." dans: Journal of immunological methods, Vol. 120, Issue 1, pp. 125-31, (1989) (PubMed).

Détail du antigène

Antigène: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Autre désignation: MCP-1

Sujet: Human monocyte chemoattractant protein-1 (MCP-1), previously known as MCAF (monocyte chemotactic and activating factor), is a member of the CC chemokine family. MCP-1 is produced by a variety of stimulated cell types including monocytes, lymphocytes, endothelial cells and fibroblasts. MCP-1 is a potent chemoattractant for monocytes and it also activates lymphocytes, basophils and NK cells.

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, Phosphorylation & l'infection par le SRAS-CoV-2