Factor VII Kit ELISA

N° du produit ABIN2702923

Détail du produit Factor VII Kit ELISA

Antigène: Factor VII (F7) (Coagulation Factor VII (F7))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.4-100 ng/mL

Seuil minimal de détection: 0.4 ng/mL

Application: ELISA

Fonction: Human Coagulation Factor VII ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificité: The antibody pair provided in this kit recognizes Human Coagulation Factor VII (F7)

Sensibilité: 0.4 ng/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples2 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: Factor VII (F7) (Coagulation Factor VII (F7))

Autre désignation: Coagulation Factor VII (F7 Produits)

Synonymes: F7 Kit ELISA, wu:fb59f04 Kit ELISA, zgc:109870 Kit ELISA, SPCA Kit ELISA, AI114908 Kit ELISA, ATIII Kit ELISA, At-3 Kit ELISA, At3 Kit ELISA, LOC100223776 Kit ELISA, AI132620 Kit ELISA, Cf7 Kit ELISA, FVII Kit ELISA, mfVII Kit ELISA, f7 Kit ELISA, coagulation factor VII Kit ELISA, serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1 Kit ELISA, coagulation factor 7 (serum prothrombin conversion accelerator) S homeolog Kit ELISA, F7 Kit ELISA, f7 Kit ELISA, Serpinc1 Kit ELISA, CpipJ_CPIJ009142 Kit ELISA, CpipJ_CPIJ010295 Kit ELISA, CpipJ_CPIJ020127 Kit ELISA, fa7 Kit ELISA, f7.S Kit ELISA

Sujet: Gene Names: F7
Protein names: Coagulation factor VII (EC 3.4.21.21) (Proconvertin) (Serum prothrombin conversion accelerator) (SPCA) (Eptacog alfa) [Cleaved into: Factor VII light chain, Factor VII heavy chain]

ID gène: 2155

UniProt: P08709

Pathways: Response to Growth Hormone Stimulus, Platelet-derived growth Factor Receptor Signaling