PTGS2 Kit ELISA

N° du produit ABIN2702925

Détail du produit PTGS2 Kit ELISA

Antigène: PTGS2 (Prostaglandin-Endoperoxide Synthase 2 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.2-300 ng/mL

Seuil minimal de détection: 1.2 ng/mL

Application: ELISA

Fonction: Human COX-2 (PTGS2) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificité: This ELISA antibody pair detects human COX-2. Other species not determined.

Sensibilité: 1.2 ng/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples2 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Références pour PTGS2 Kit ELISA (ABIN2702925)

Fawzy, Elfayoumi, Mohamed, Fatah, Saadawy: "Cyclooxygenase 2 (rs2745557) Polymorphism and the Susceptibility to Benign Prostate Hyperplasia and Prostate Cancer in Egyptians." dans: Biochemical genetics, Vol. 54, Issue 3, pp. 326-36, (2017) (PubMed).

Said, Smith, Sanchez-Carbayo, Theodorescu: "Tumor endothelin-1 enhances metastatic colonization of the lung in mouse xenograft models of bladder cancer." dans: The Journal of clinical investigation, Vol. 121, Issue 1, pp. 132-47, (2011) (PubMed).

Détail du antigène

Antigène: PTGS2 (Prostaglandin-Endoperoxide Synthase 2 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS2))

Autre désignation: COX-2 (PTGS2 Produits)

Synonymes: COX-2 Kit ELISA, COX2 Kit ELISA, GRIPGHS Kit ELISA, PGG/HS Kit ELISA, PGHS-2 Kit ELISA, PHS-2 Kit ELISA, hCox-2 Kit ELISA, Cox-2 Kit ELISA, Pghs2 Kit ELISA, TIS10 Kit ELISA, Cox2 Kit ELISA, cox-2 Kit ELISA, CEF147 Kit ELISA, PGHS2 Kit ELISA, PHSII Kit ELISA, cox2 Kit ELISA, gripghs Kit ELISA, pgg/hs Kit ELISA, pghs-2 Kit ELISA, phs-2 Kit ELISA, PTGS2 Kit ELISA, hcox-2 Kit ELISA, fj02a10 Kit ELISA, ptgs2 Kit ELISA, unp1239 Kit ELISA, wu:fj02a10 Kit ELISA, zCOX-2 Kit ELISA, si:dkey-97o5.6 Kit ELISA, prostaglandin-endoperoxide synthase 2 Kit ELISA, prostaglandin G/H synthase 2 Kit ELISA, prostaglandin-endoperoxide synthase 2S homeolog Kit ELISA, prostaglandin-endoperoxide synthase 2a Kit ELISA, prostaglandin-endoperoxide synthase 2b Kit ELISA, PTGS2 Kit ELISA, Ptgs2 Kit ELISA, LOC100136456 Kit ELISA, ptgs2.S Kit ELISA, ptgs2 Kit ELISA, ptgs2a Kit ELISA, ptgs2b Kit ELISA

Sujet: Gene Names: PTGS2 COX2
Protein names: Prostaglandin G/H synthase 2 (EC 1.14.99.1) (Cyclooxygenase-2) (COX-2) (PHS II) (Prostaglandin H2 synthase 2) (PGH synthase 2) (PGHS-2) (Prostaglandin-endoperoxide synthase 2)

ID gène: 5743

UniProt: P35354

Pathways: Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation