FABP2 Kit ELISA

N° du produit ABIN4882785

Détail du produit FABP2 Kit ELISA

Antigène: FABP2 (Fatty Acid Binding Protein 2, Intestinal (FABP2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 25 pg/mL-100 ng/mL

Seuil minimal de détection: 25 pg/mL

Application: ELISA

Fonction: Human FABP2 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: In this ELISA antibody pair format, no cross-reactivity was observed with rhFABP-1, 3, 5, 6, 7, 8 and 9.

Sensibilité: 25 pg/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples2 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Références pour FABP2 Kit ELISA (ABIN4882785)

Ji, Xie, Yin, Qi, Chen, Bai, Wang, Zhao, Jiang, Jiang: "Bile acid receptor TGR5 overexpression is associated with decreased intestinal mucosal injury and epithelial cell proliferation in obstructive jaundice." dans: Translational research : the journal of laboratory and clinical medicine, Vol. 182, pp. 88-102, (2017) (PubMed).

Détail du antigène

Antigène: FABP2 (Fatty Acid Binding Protein 2, Intestinal (FABP2))

Autre désignation: FABP2 (FABP2 Produits)

Synonymes: I-FABP Kit ELISA, ifabp Kit ELISA, zgc:92264 Kit ELISA, IFABP Kit ELISA, fabpi Kit ELISA, i-fabp Kit ELISA, fabp2-A Kit ELISA, FABP2 Kit ELISA, FABPI Kit ELISA, FABP Kit ELISA, Fabpi Kit ELISA, fabp2 Kit ELISA, fabp2a Kit ELISA, fatty acid binding protein 2, intestinal Kit ELISA, fatty acid binding protein 2 Kit ELISA, fatty acid binding protein 2 L homeolog Kit ELISA, fabp2 Kit ELISA, FABP2 Kit ELISA, Fabp2 Kit ELISA, fabp2.L Kit ELISA

Sujet: Gene Names: FABP2 FABPI
Protein names: Fatty acid-binding protein, intestinal (Fatty acid-binding protein 2) (Intestinal-type fatty acid-binding protein) (I-FABP)

ID gène: 2169

UniProt: P12104