SPINT2 Kit ELISA

N° du produit ABIN2703071

Détail du produit SPINT2 Kit ELISA

Antigène: SPINT2 (serine Peptidase Inhibitor, Kunitz Type, 2 (SPINT2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 3-800 pg/mL

Seuil minimal de détection: 3 pg/mL

Application: ELISA

Fonction: Human HAI-2 ELISA Kit for cell culture supernatants, EDTA and/or Citrate treated plasma, and serum samples. Heparin is not recommended as an anticoagulant for use in this assay.

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificité: This ELISA antibody pair detects human HAI-2, less than 0.1 % cross-reactivity with human HAI-1 and mouse HAI-2B is observed

Sensibilité: 3 pg/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples2 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Préparation de l'échantillon:

Collect plasma using EDTA and/or Citrate as anticoagulants. Heparin is not recommended as an anticoagulant for use in this assay.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: SPINT2 (serine Peptidase Inhibitor, Kunitz Type, 2 (SPINT2))

Autre désignation: HAI-2 (SPINT2 Produits)

Synonymes: HAI-2 Kit ELISA, Pb Kit ELISA, DIAR3 Kit ELISA, HAI2 Kit ELISA, Kop Kit ELISA, PB Kit ELISA, AL024025 Kit ELISA, C76321 Kit ELISA, serine peptidase inhibitor, Kunitz type, 2 Kit ELISA, serine peptidase inhibitor, Kunitz type 2 Kit ELISA, serine protease inhibitor, Kunitz type 2 Kit ELISA, Spint2 Kit ELISA, SPINT2 Kit ELISA

Sujet: Gene Names: SPINT2 HAI2 KOP
Protein names: Kunitz-type protease inhibitor 2 (Hepatocyte growth factor activator inhibitor type 2) (HAI-2) (Placental bikunin)

ID gène: 10653

UniProt: O43291