Insulin Kit ELISA

N° du produit ABIN2703271

Détail du produit Insulin Kit ELISA

Antigène: Insulin (INS)

Reactivité: Humain, Rat, Souris, Porc, Boeuf (Vache)

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 4-300 μIU/mL

Seuil minimal de détection: 4 μIU/mL

Application: ELISA

Fonction: Porcine Insulin ELISA Kit for cell culture supernatants, plasma, and serum samples.

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificité: This ELISA antibody pair detects porcine, mouse, human, rat, and bovine Insulin.

Sensibilité: 4 μIU/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples2 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: Insulin (INS)

Autre désignation: Insulin (INS Produits)

Synonymes: IDDM2 Kit ELISA, ILPR Kit ELISA, IRDN Kit ELISA, MODY10 Kit ELISA, ins1 Kit ELISA, xins Kit ELISA, ins1-a Kit ELISA, Insulin Kit ELISA, AA986540 Kit ELISA, Ins-2 Kit ELISA, InsII Kit ELISA, Mody Kit ELISA, Mody4 Kit ELISA, proinsulin Kit ELISA, zgc:109842 Kit ELISA, igf2-A Kit ELISA, ins Kit ELISA, ins-a Kit ELISA, ins-b Kit ELISA, insulin Kit ELISA, insulin precursor Kit ELISA, insulin II Kit ELISA, preproinsulin Kit ELISA, insulin L homeolog Kit ELISA, insulin S homeolog Kit ELISA, INS Kit ELISA, INS-IGF2 Kit ELISA, ins Kit ELISA, Ins Kit ELISA, PIN Kit ELISA, Ins2 Kit ELISA, ins.L Kit ELISA, ins.S Kit ELISA

Sujet: Gene Names: INS
Protein names: Insulin [Cleaved into: Insulin B chain, Insulin A chain]

ID gène: 397415

UniProt: P01315

Pathways: Signalisation NF-kappaB, Signalisation RTK, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation