PD-1 Kit ELISA

N° du produit ABIN2703394

Détail du produit PD-1 Kit ELISA

Antigène: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 20-6.000 pg/mL

Seuil minimal de détection: 20 pg/mL

Application: ELISA

Fonction: Human PD-1 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificité: This ELISA antibody pair detects human PD-1. Other species not determined yet.

Sensibilité: 20 pg/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples2 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Références pour PD-1 Kit ELISA (ABIN2703394)

Yanaba, Hayashi, Yoshihara, Nakagawa: "Serum levels of soluble programmed death-1 and programmed death ligand-1 in systemic sclerosis: Association with extent of skin sclerosis." dans: The Journal of dermatology, Vol. 43, Issue 8, pp. 954-7, (2016) (PubMed).

Détail du antigène

Antigène: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Autre désignation: PD-1 (PDCD1 Produits)

Synonymes: CD279 Kit ELISA, PD-1 Kit ELISA, PD1 Kit ELISA, SLEB2 Kit ELISA, hPD-1 Kit ELISA, hPD-l Kit ELISA, Ly101 Kit ELISA, Pdc1 Kit ELISA, programmed cell death 1 Kit ELISA, PDCD1 Kit ELISA, Pdcd1 Kit ELISA

Sujet: Gene Names: PDCD1 PD1
Protein names: Programmed cell death protein 1 (Protein PD-1) (hPD-1) (CD antigen CD279)

ID gène: 5133

UniProt: Q15116

Pathways: Cancer Immune Checkpoints