×
Pour une meilleure utilisation, nous vous conseillons d’activer Javascript dans votre navigateur.
SERPINA1 Kit ELISA
SERPINA1
Reactivité: Humain
Colorimetric
Sandwich ELISA
2-500 ng/mL
Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN2703451
Détail du produit SERPINA1 Kit ELISA
(cache)
Antigène
Voir toutes SERPINA1 Kits ELISA
SERPINA1
(serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1))
Reactivité
Toutes les réactivités sur SERPINA1 Kits ELISA
Humain
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Gamme de detection
2-500 ng/mL
Seuil minimal de détection
2 ng/mL
Application
ELISA
Fonction
Human SERPINA1 (Alpha-1-Antitrypsin) ELISA Kit for cell culture supernatants, plasma, and serum samples.
Type d'échantillon
Cell Culture Supernatant, Plasma, Serum
Analytical Method
Quantitative
Specificité
This ELISA antibody pair detects human Serpin A1. Other species not determined.
Sensibilité
2 ng/mL
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Featured
Discover our best selling SERPINA1 Kit ELISA
Top Product
Discover our top product SERPINA1 Kit ELISA
Alternatives
(show)
Information d'application
(cache)
Indications d'application
Recommended Dilution for serum and plasma samples3-30 fold
Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
(cache)
Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
(cache)
Antigène
Voir toutes SERPINA1 Kits ELISA
SERPINA1
(serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1))
Autre désignation
Serpin A1 (SERPINA1 Produits )
Synonymes
AACT Kit ELISA, ACT Kit ELISA, GIG25 Kit ELISA, A1AT Kit ELISA, PI Kit ELISA, alpha-1-PI Kit ELISA, A1A Kit ELISA, AAT Kit ELISA, PI1 Kit ELISA, PRO2275 Kit ELISA, alpha1AT Kit ELISA, Pi Kit ELISA, Spi1 Kit ELISA, MGC64429 Kit ELISA, serpina1d Kit ELISA, Aps Kit ELISA, SERPINA3-5 Kit ELISA, serpin family A member 3 Kit ELISA, alpha-1-antiproteinase F precursor Kit ELISA, serpin family A member 1 Kit ELISA, alpha-1-antitrypsin Kit ELISA, calumenin Kit ELISA, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 S homeolog Kit ELISA, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 Kit ELISA, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 5 Kit ELISA, SH2B adaptor protein 2 Kit ELISA, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3 Kit ELISA, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 L homeolog Kit ELISA, SERPINA3 Kit ELISA, LOC100008973 Kit ELISA, SERPINA1 Kit ELISA, LOC100328621 Kit ELISA, CALU Kit ELISA, Serpina1 Kit ELISA, serpina1.S Kit ELISA, serpina1 Kit ELISA, SPIA5 Kit ELISA, Sh2b2 Kit ELISA, Calu Kit ELISA, serpina1.L Kit ELISA
Sujet
Gene Names: SERPINA1 AAT PI PRO0684 PRO2209 Protein names: Alpha-1-antitrypsin (Alpha-1 protease inhibitor) (Alpha-1-antiproteinase) (Serpin A1) [Cleaved into: Short peptide from AAT (SPAAT)]
ID gène
5265
UniProt
P01009
Vus récemment
(cache)