EIF2B1 Kit ELISA
-
- Antigène Voir toutes EIF2B1 Kits ELISA
- EIF2B1 (Eukaryotic Translation Initiation Factor 2B, Subunit 1 Alpha, 26kDa (EIF2B1))
- Épitope
- pSer52
- Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Application
- ELISA
- Fonction
- Human Phospho-EIF2A (S52) ELISA Kit. This assay semi-quantitatively measures phophorylated EIF2A (Ser52) in lysate samples.
- Type d'échantillon
- Cell Lysate, Tissue Lysate
- Analytical Method
- Semi-Quantitative
- Specificité
- The antibody pair provided in this kit recognizes human eIF-2A phosphorylated at site Serine-52.
- Attributs du produit
-
- Rapidly measure phosphorylated protein in lysates
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
- Ingrédients
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- HRP-Conjugated Secondary Antibody
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
- Matériel non inclus
-
- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
-
-
- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Protocole
-
- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Procédure de l'essai
-
Prepare all reagents and samples as instructed in the manual.
Add 100 μL of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately. - Restrictions
- For Research Use only
-
- Stock
- -20 °C
- Stockage commentaire
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- Date de péremption
- 6 months
-
- Antigène Voir toutes EIF2B1 Kits ELISA
- EIF2B1 (Eukaryotic Translation Initiation Factor 2B, Subunit 1 Alpha, 26kDa (EIF2B1))
- Autre désignation
- eIF-2a (EIF2B1 Produits)
- Synonymes
- DDBDRAFT_0219393 Kit ELISA, DDBDRAFT_0231375 Kit ELISA, DDB_0219393 Kit ELISA, DDB_0231375 Kit ELISA, 26kDa Kit ELISA, D5Ertd406e Kit ELISA, EIF2B Kit ELISA, EIF2BA Kit ELISA, eIF-2a Kit ELISA, translation initiation factor eIF-2B alpha subunit Kit ELISA, hypothetical protein Kit ELISA, Translation initiation factor eIF-2B subunit alpha Kit ELISA, eukaryotic translation initiation factor 2B subunit alpha Kit ELISA, eukaryotic translation initiation factor 2B, subunit 1 (alpha) Kit ELISA, eIF2b1 Kit ELISA, PGTG_05021 Kit ELISA, ei2ba Kit ELISA, eif2b1 Kit ELISA, Eif2b1 Kit ELISA, EIF2B1 Kit ELISA
- Sujet
- Eukaryotic translation initiation factor 2A (eIF-2A) phosphorylated at Serine-52
- ID gène
- 1965
- UniProt
- P05198
- Pathways
- Methionine Biosynthetic Process
-