PTK2B Kit ELISA (PTK2B Protein tyrosine Kinase 2 beta)

Details for Product PTK2B ELISA Kit No. ABIN2748576
Antigène
  • CADTK
  • CAKB
  • FADK2
  • FAK2
  • PKB
  • PTK
  • PYK2
  • RAFTK
  • CAKbeta
  • E430023O05Rik
  • Raftk
  • Pyk2
  • fak1b
  • ptk2.2
  • ptk2b
  • si:ch1073-355e8.1
  • protein tyrosine kinase 2 beta
  • PTK2 protein tyrosine kinase 2 beta
  • protein tyrosine kinase 2aa
  • PTK2B
  • Ptk2b
  • ptk2aa
Épitope
pTyr402
Reactivité
Humain
Alternatives
Kits with alternative reactivity to:
9
5
5
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Options
Fonction Human Phospho-PYK2 (Y402) ELISA Kit. This assay semi-quantitatively measures phophorylated PYK2 (Tyr402) in lysate samples.
Type d'échantillon Cell Lysate, Tissue Lysate
Analytical Method Semi-Quantitative
Méthode de détection Colorimetric
Specificité The antibody pair provided in this kit recognizes PYK2 phosphorylated at Tyrosine-402.
Attributs du produit
  • Rapidly measure phosphorylated protein in lysates
  • Screen numerous different cell lysates without performing a Western Blot analysis
  • Minimal hands-on time, convenient, and non-radioactive material
Ingrédients
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Anti-Phospho Antibody
  • HRP-Conjugated Secondary Antibody
  • Assay Diluent
  • TMB One-Step Substrate
  • Stop Solution
  • Lysis Buffer
  • Positive Control Sample
Matériel non inclus
  • Distilled or deionized water
  • 100 mL and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 μL to 1 mL volumes
  • Adjustable 1-25 mL pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm
Plasmids, Primers & others Plasmids, Primers & others PTK2B products on genomics-online (e.g. as negative or positive controls)
Antigène
Autre désignation PYK2 (PTK2B ELISA Kit Extrait)
Sujet Protein tyrosine kinase 2 beta, PTK2B, CADTK, CAKB, FADK2, FAK2, PKB, PTK, PYK2, RAFTK
ID gène 2185
UniProt Q14289
Pathways EGFR Signaling Pathway, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Cell-Cell Junction Organization, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGF Signaling
Plaque Pre-coated
Protocole
  1. Prepare all reagents and samples as instructed in the manual.
  2. Add 100 μL of sample or positive control to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared primary antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
  7. Incubate 1 h at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Procédure de l'essai

Prepare all reagents and samples as instructed in the manual.Add 100 μL of sample or positive control to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared primary antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared 1X HRP-Streptavidin to each well.Incubate 1 h at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

Restrictions For Research Use only
Stock -20 °C
Stockage commentaire Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
Date de péremption 6 months
Images (Fournisseur)
ELISA image for PTK2B Protein tyrosine Kinase 2 beta (PTK2B) ELISA Kit (ABIN2748576) JURKAT cells were treated with Pervanadate at 37°C for 10 min. Cells were solubil...
ELISA image for PTK2B Protein tyrosine Kinase 2 beta (PTK2B) ELISA Kit (ABIN2748576) JURKAT cells were untreated or treated with Pervanadate for 10 min. Cell lysates were...
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