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PTK2B Kit ELISA

PTK2B Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6730589
  • Antigène Voir toutes PTK2B Kits ELISA
    PTK2B (PTK2B Protein tyrosine Kinase 2 beta (PTK2B))
    Reactivité
    • 6
    • 4
    • 4
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human PYK2/FAK2 ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair recognizes Human PYK2.
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product PTK2B Kit ELISA
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes PTK2B Kits ELISA
    PTK2B (PTK2B Protein tyrosine Kinase 2 beta (PTK2B))
    Autre désignation
    PYK2 (FAK2 (PTK2B Produits)
    Synonymes
    CADTK Kit ELISA, CAKB Kit ELISA, FADK2 Kit ELISA, FAK2 Kit ELISA, PKB Kit ELISA, PTK Kit ELISA, PYK2 Kit ELISA, RAFTK Kit ELISA, CAKbeta Kit ELISA, E430023O05Rik Kit ELISA, Raftk Kit ELISA, Pyk2 Kit ELISA, fak1b Kit ELISA, ptk2.2 Kit ELISA, ptk2b Kit ELISA, si:ch1073-355e8.1 Kit ELISA, protein tyrosine kinase 2 beta Kit ELISA, PTK2 protein tyrosine kinase 2 beta Kit ELISA, protein tyrosine kinase 2aa Kit ELISA, PTK2B Kit ELISA, Ptk2b Kit ELISA, ptk2aa Kit ELISA
    UniProt
    Q14289
    Pathways
    EGFR Signaling Pathway, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Cell-Cell Junction Organization, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGF Signaling
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