VEGFR2/CD309 Kit ELISA
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- Antigène Voir toutes VEGFR2/CD309 (VEGFR2) Kits ELISA
- VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))
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Reactivité
- Macaque rhésus
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Application
- ELISA
- Fonction
- Custom Rhesus Macaque Vascular Endothelial Growth Factor Receptor 2 (VEGFR2) ELISA Kit.
- Type d'échantillon
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- Specificité
- The antibody pair provided in this kit recognizes Rhesus Macaque Vascular Endothelial Growth Factor Receptor 2 (VEGFR2)
- Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Procédure de l'essai
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Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Stock
- -20 °C
- Stockage commentaire
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Date de péremption
- 6 months
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- Antigène Voir toutes VEGFR2/CD309 (VEGFR2) Kits ELISA
- VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))
- Autre désignation
- VEGFR2 (VEGFR2 Produits)
- Synonymes
- CD309 Kit ELISA, FLK1 Kit ELISA, VEGFR Kit ELISA, VEGFR2 Kit ELISA, 6130401C07 Kit ELISA, Flk-1 Kit ELISA, Flk1 Kit ELISA, Krd-1 Kit ELISA, Ly73 Kit ELISA, VEGFR-2 Kit ELISA, sVEGFR-2 Kit ELISA, Vegfr-2 Kit ELISA, FLK-1 Kit ELISA, flk-1 Kit ELISA, vegfr-2 Kit ELISA, KDR Kit ELISA, FLK/KDR Kit ELISA, flk1 Kit ELISA, flk1b Kit ELISA, kdrb Kit ELISA, si:busm1-205d10.1 Kit ELISA, si:ch211-254j6.1 Kit ELISA, si:ch211-278f21.4 Kit ELISA, wu:fc31a09 Kit ELISA, fk52c05 Kit ELISA, flk Kit ELISA, kdr Kit ELISA, kdra Kit ELISA, vegfr2 Kit ELISA, vegfr4 Kit ELISA, vegr2 Kit ELISA, wu:fk52c05 Kit ELISA, kinase insert domain receptor Kit ELISA, kinase insert domain protein receptor Kit ELISA, vascular endothelial growth factor receptor kdr-like Kit ELISA, kinase insert domain receptor (a type III receptor tyrosine kinase) Kit ELISA, kinase insert domain receptor like Kit ELISA, KDR Kit ELISA, Kdr Kit ELISA, LOC100136647 Kit ELISA, kdr Kit ELISA, kdrl Kit ELISA
- Sujet
- Vascular Endothelial Growth Factor Receptor 2 (VEGFR2)
- UniProt
- F7E313
- Pathways
- Signalisation RTK, Glycosaminoglycan Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling
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