NBL1 Kit ELISA

N° du produit ABIN2859290

Détail du produit NBL1 Kit ELISA

Antigène: NBL1 (Neuroblastoma 1, DAN Family BMP Antagonist (NBL1))

Épitope: AA 17-178

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5-4000 pg/mL

Seuil minimal de détection: 62.5 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse DAN/NBL1

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: NSO, A17-D178

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: A17-D178

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plaque: Pre-coated

Protocole: mouse DAN ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for DAN has been precoated onto 96-well plates. Standards (NSO, A17-D178) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for DAN is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse DAN amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse DAN standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse DAN standard solution and each sample is measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 675, Standard deviation: 37.12, CV(%): 5.5
• Sample 2: n=16, Mean(pg/ml): 1810, Standard deviation: 112.22, CV(%): 6.2
• Sample 3: n=16, Mean(pg/ml): 2944, Standard deviation: 197.24, CV(%): 6.7,
• Sample 1: n=24, Mean(pg/ml): 792, Standard deviation: 50.68, CV(%): 6.4
• Sample 2: n=24, Mean(pg/ml): 1948, Standard deviation: 146.10, CV(%): 7.5
• Sample 3: n=24, Mean(pg/ml): 3107, Standard deviation: 242.34, CV(%): 7.8

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: NBL1 (Neuroblastoma 1, DAN Family BMP Antagonist (NBL1))

Autre désignation: NBL1 (NBL1 Produits)

Synonymes: D1S1733E Kit ELISA, DAN Kit ELISA, DAND1 Kit ELISA, NB Kit ELISA, NO3 Kit ELISA, D4H1S1733E Kit ELISA, Dana Kit ELISA, dand1 Kit ELISA, nbl1-a Kit ELISA, no3 Kit ELISA, 4.1N Kit ELISA, Epb41l1 Kit ELISA, NBL1 Kit ELISA, mKIAA0338 Kit ELISA, C1orf151-NBL1 Kit ELISA, dan Kit ELISA, zgc:77251 Kit ELISA, neuroblastoma 1, DAN family BMP antagonist Kit ELISA, neuroblastoma, suppression of tumorigenicity 1 Kit ELISA, neuroblastoma 1, DAN family BMP antagonist L homeolog Kit ELISA, neuroblastoma suppressor of tumorigenicity 1 Kit ELISA, erythrocyte membrane protein band 4.1 like 1 Kit ELISA, MINOS1-NBL1 readthrough Kit ELISA, NBL1 Kit ELISA, Nbl1 Kit ELISA, nbl1.L Kit ELISA, LOC535280 Kit ELISA, Epb41l1 Kit ELISA, MINOS1-NBL1 Kit ELISA, nbl1 Kit ELISA

Sujet:

Protein Function: Possible candidate as a tumor suppressor gene of neuroblastoma. May play an important role in preventing cells from entering the final stage (G1/S) of the transformation process.

Background: Differential screening-selected gene aberrative in neuroblastoma (DAN) is a member of the DAN family of secreted glycoproteins that are putative BMP antagonists. The NBL1 gene, also known as DAN, is originally cloned from a normal rat fibroblast cDNA library by a differential screening method. The human DAN gene is mapped to chromosome 1p36.13-p36. It is found that the DAN gene possesses a tumor suppressive activity when overexpressed in v-src transformed cells.

Synonyms: Neuroblastoma suppressor of tumorigenicity 1,N03,Zinc finger protein DAN,Nbl1,Dan, Dana,

Full Gene Name: Neuroblastoma suppressor of tumorigenicity 1

Cellular Localisation: Secreted.

ID gène: 17965

UniProt: Q61477