FOXO1 Kit ELISA

N° du produit ABIN2866265

Kit ELISA Humain FOXO1, test Colorimetric pour la quantification de Humain FOXO1.

Aperçu rapide pour FOXO1 Kit ELISA (ABIN2866265)

Antigène: FOXO1 (Forkhead Box O1 (FOXO1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: DNA-Binding ELISA

Application: ELISA

Type d'échantillon: Cell Extracts, Tissue Samples

Détail du produit FOXO1 Kit ELISA

Fonction: DNA-binding ELISA that facilitate the study of transcription factor activation in mammalien tissue and cell culture extracts.

Marque: TransAM®

Analytical Method: Quantitative

Specificité: TransAM FKHR (FOXO1/4) Kits are tested for sensitivity in detecting FKHR activation.

Attributs du produit: Transcription factors are DNA-binding proteins that tightly regulate gene expression. They consist of two distinct domains - one that displays high affinity for a specific DNA sequence and one that confers transcriptional activity. Transcription factors are activated by phosphorylation of specific residues or by processing bound inhibitory proteins. Understanding and quantifying transcription factors is essential for the study of cell functions in relation to differentiation, brain activity, immune response, inflammation and various disease states. TransAM® Kits are sensitive, non-radioactive transcription factor ELISA kits that facilitate the study of transcription factor activation in mammalian tissue and cell extracts.
TransAM® Kits are DNA-binding ELISAs that facilitate the study of transcription factor activation in mammalian tissue and cell extracts. Each kit includes a 96-stripwell plate in which multiple copies of a specific double-stranded oligonucleotide have been immobilized. When nuclear or whole-cell extract is added, activated transcription factor of interest binds the oligonucleotide at its consensus binding site and is quantified using the included antibody, which is specific for the bound, active form of the transcription factor being studied.

Ingrédients: One or five 96-well plate(s) with plate sealer(s), primary antibody, HRP-conjugated secondary antibody, wild-type and mutated competitor oligonucleotides, positive control cell extract, DTT, Protease Inhibitor Cocktail, Lysis, Binding, 10X Washing and 10X Antibody Binding Buffers, and Developing and Stop Solutions.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Nuclear extracts prepared from Raji and HeLa cells are diluted to 1.2 μg/well and assayed using the TransAM FKHR (FOXO1/4) Kit. The ratio of the signals from the Raji cells over the HeLa cells must be above 5. Lot No. 09814003 was developed for 12 minutes. It gave a ratio of 5.72(Figure 1). The endogenous level of FKHR expression, and this ratio may vary depending on the cell type tested and the treatment used. TransAM FKHR (FOXO1/4) Kits are also tested for specificity in detecting FKHR (FOXO1/4) activity. TransAM FKHR (FOXO1/4) assays are performed in the presence of an excess of oligonucleotide containing a wild-type or mutated FKHR consensus binding site (Figure 2). At 10X excess, the wild-type oligonucleotide prevents FKHR binding to the probe immobilized on the plate. Conversely, the mutated oligonucleotide has no effect.

Durée du test: 5 h

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C/-80 °C

Stockage commentaire: Store the cell extract at -80°C. Other kit components can be stored at -20°C prior to first use. Then, we recommend storing the kit at 4°C except for the primary antibody, oligonucleotides, DTT and Protease Inhibitor Cocktail that should be kept at -20°C. This product is guaranteed for 6 months from date of receipt.

Date de péremption: 6 months

Détail du antigène

Antigène: FOXO1 (Forkhead Box O1 (FOXO1))

Autre désignation: Fkhr

Pathways: Signalisation PI3K-Akt, Cycle Cellulaire, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Carbohydrate Metabolic Process, CXCR4-mediated Signaling Events, BCR Signaling