TNF alpha Kit ELISA

N° du produit ABIN414928

Kit ELISA TNF alpha Humain, Colorimetric test pour la quantification de Humain TNF alpha et a été mentionné dans 60+ publications.

Aperçu rapide pour TNF alpha Kit ELISA (ABIN414928)

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6 pg/mL - 1000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Détail du produit TNF alpha Kit ELISA

Seuil minimal de détection: 15.6 pg/mL

Fonction: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TNFa in Serum,Plasma,Tissue Homogenate,Cell Lysate,Cell Culture Supernatant,Biological Fluids

Analytical Method: Quantitative

Specificité:

This assay has high sensitivity and excellent specificity for detection of Tumor Necrosis Factor Alpha (TNFa).
No significant cross-reactivity or interference between Tumor Necrosis Factor Alpha (TNFa) and analogues was observed.

Réactivité croisée (Details): No significant cross-reactivity or interference between Tumor Necrosis Factor Alpha (TNFa) and analogues was observed.

Sensibilité: < 6.4 pg/mL

Ingrédients:

• Pre-coated, 96-well strip plate
• Plate sealer
• Standard
• Standard Diluent
• Detection Reagent A
• Assay Diluent A
• Detection Reagent B
• Assay Diluent B
• TMB Substrate
• Stop Solution
• Wash Buffer (30X)
• Instruction manual

Matériel non inclus:

• Microplate reader with 450 nm filter.
• Precision single or multi-channel pipettes and disposable tips.
• Eppendorf Tubes for diluting samples.
• Deionized or distilled water.
• Absorbent paper for blotting the microtiter plate.
• Container for Wash Solution

Information d'application

Indications d'application:

• Limited by the current condition and scientific technology, we cannot completely conduct the comprehensive identification and analysis on the raw material provided by suppliers. So there might be some qualitative and technical risks to use the kit.
• The final experimental results will be closely related to validity of the products, operation skills of the end users and the experimental environments. Please make sure that sufficient samples are available.
• Kits from different batches may be a little different in detection range, sensitivity and color developing time.
• Do not mix or substitute reagents from one kit lot to another. Use only the reagents supplied by manufacturer.
• Protect all reagents from strong light during storage and incubation. All the bottle caps of reagents should be covered tightly to prevent the evaporation and contamination of microorganism.
• There may be some foggy substance in the wells when the plate is opened at the first time. It will not have any effect on the final assay results. Do not remove microtiter plate from the storage bag until needed.
• Wrong operations during the reagents preparation and loading, as well as incorrect parameter setting for the plate reader may lead to incorrect results. A microplate plate reader with a bandwidth of 10nm or less and an optical density range of 0-3 O.D. or greater at 450 ± 10nm wavelength is acceptable for use in absorbance measurement. Please read the instruction carefully and adjust the instrument prior to the experiment.
• Even the same operator might get different results in two separate experiments. In order to get better reproducible results, the operation of every step in the assay should be controlled. Furthermore, a preliminary experiment before assay for each batch is recommended.
• Each kit has been strictly passed Q.C test. However, results from end users might be inconsistent with our in-house data due to some unexpected transportation conditions or different lab equipments. Intra-assay variance among kits from different batches might arise from above factors, too.
• Kits from different manufacturers for the same item might produce different results, since we have not compared our products with other manufacturers.

Commentaires:

Information on standard material:
The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

Information on reagents:
The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

Information on antibodies:
The provided antibodies and their host vary in different kits.

Volume d'échantillon: 100 μL

Durée du test: 3 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

Précision du teste:

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tumor Necrosis Factor Alpha (TNFa) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tumor Necrosis Factor Alpha (TNFa) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Précaution d'utilisation: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Conseil sur la manipulation: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Stock: 4 °C

Stockage commentaire:

• For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
• For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
  Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
• For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.

Date de péremption: 6 months

Références pour TNF alpha Kit ELISA (ABIN414928)

Feng, Wei, Liang, Sun, Duan: "miR-205/IRAK2 signaling pathway is associated with urban airborne PM2.5-induced myocardial toxicity." dans: Nanotoxicology, Vol. 14, Issue 9, pp. 1198-1212, (2021) (PubMed).

Zhang, Wu, Li, Wang, Fang, Liu: "Melatonin enhances autophagy and decreases apoptosis induced by nanosilica in RAW264.7 cells." dans: IUBMB life, Vol. 71, Issue 7, pp. 1021-1029, (2020) (PubMed).

Gibbs, Silverman, Zhao, Peng, Wang, Wellington, Mackenzie, Plotkin, Kaplan, Cashman: "A Rationally Designed Humanized Antibody Selective for Amyloid Beta Oligomers in Alzheimer's Disease." dans: Scientific reports, Vol. 9, Issue 1, pp. 9870, (2020) (PubMed).

Sun, Han, Cheng, Zheng, Xiao, So, Zhang: "Corticosterone-mediated microglia activation affects dendritic spine plasticity and motor learning functions in minimal hepatic encephalopathy." dans: Brain, behavior, and immunity, Vol. 82, pp. 178-187, (2020) (PubMed).

Park, Kim, Kim, Kang, Park, Ahn, Nam: "Supplementation with Korean Red Ginseng Improves Current Perception Threshold in Korean Type 2 Diabetes Patients: A Randomized, Double-Blind, Placebo-Controlled Trial." dans: Journal of diabetes research, Vol. 2020, pp. 5295328, (2020) (PubMed).

Bassani, Bonato, Machado, Cóppola-Segovia, Moura, Zanata, Oliveira, Vital: "Decrease in Adult Neurogenesis and Neuroinflammation Are Involved in Spatial Memory Impairment in the Streptozotocin-Induced Model of Sporadic Alzheimer's Disease in Rats." dans: Molecular neurobiology, Vol. 55, Issue 5, pp. 4280-4296, (2019) (PubMed).

Velusami, Richard, Bethapudi: "Polar extract of Curcuma longa protects cartilage homeostasis: possible mechanism of action." dans: Inflammopharmacology, Vol. 26, Issue 5, pp. 1233-1243, (2019) (PubMed).

Song, Park, Lee, Lee: "Amniotic fluid HIF1α and exosomal HIF1α in cervical insufficiency patients with physical examination-indicated cerclage." dans: The journal of maternal-fetal & neonatal medicine, Vol. 32, Issue 14, pp. 2287-2294, (2019) (PubMed).

Guo, Wang, Wang, Qiao, Tang: "Pantoprazole blocks the JAK2/STAT3 pathway to alleviate skeletal muscle wasting in cancer cachexia by inhibiting inflammatory response." dans: Oncotarget, Vol. 8, Issue 24, pp. 39640-39648, (2018) (PubMed).

Zhang, Hu, Sun, Li, Liu, Song: "Inhibitory Effect of Low-Intensity Pulsed Ultrasound on the Expression of Lipopolysaccharide-Induced Inflammatory Factors in U937 Cells." dans: Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine, Vol. 36, Issue 12, pp. 2419-2429, (2018) (PubMed).

Hu, Chen, Kan, Zhuang, Zhang, Han: "Inhibition of Fusarium solani Infection in Murine Keratocytes by Lactobacillus salivarius ssp. salivarius JCM1231 Culture Filtrate In Vitro." dans: Current eye research, Vol. 42, Issue 10, pp. 1339-1347, (2018) (PubMed).

Štofilová, Langerholc, Botta, Treven, Gradišnik, Salaj, Šoltésová, Bertková, Hertelyová, Bomba: "Cytokine production in vitro and in rat model of colitis in response to Lactobacillus plantarum LS/07." dans: Biomedicine & pharmacotherapy, Vol. 94, pp. 1176-1185, (2018) (PubMed).

Zaccaria, Curti, Di Lorenzo, Baldi, Maccario, Sommatis, Mocchi, Daglia: "Effect of Green and Brown Propolis Extracts on the Expression Levels of microRNAs, mRNAs and Proteins, Related to Oxidative Stress and Inflammation." dans: Nutrients, Vol. 9, Issue 10, (2018) (PubMed).

Wang, Wang, Deng, Jin, Jiang, Meng, Xu, Zhao, Sun, Qian: "Chronic Osteomyelitis Increases the Incidence of Type 2 Diabetes in Humans and Mice." dans: International journal of biological sciences, Vol. 13, Issue 9, pp. 1192-1202, (2018) (PubMed).

Sun, Jiang, Wang, Liu, Liu, Song, Guo, Guo, Du, Jiang, Li, Qiu, Zhao, Zhou: "Treatment of Paraquat-Induced Lung Injury With an Anti-C5a Antibody: Potential Clinical Application." dans: Critical care medicine, Vol. 46, Issue 5, pp. e419-e425, (2018) (PubMed).

Ramachandran, Anandan, Kutty, Mullasari, Pillai, Kartha: "Metformin attenuates effects of cyclophilin A on macrophages, reduces lipid uptake and secretion of cytokines by repressing decreased AMPK activity." dans: Clinical science (London, England : 1979), Vol. 132, Issue 6, pp. 719-738, (2018) (PubMed).

Bi, Sun, Wu, Chen, Tang, Mao: "PPARγ alleviated hepatocyte steatosis through reducing SOCS3 by inhibiting JAK2/STAT3 pathway." dans: Biochemical and biophysical research communications, Vol. 498, Issue 4, pp. 1037-1044, (2018) (PubMed).

Luo, Wang, Liu, Yin, A, Huang, Ye: "Inhibition of SOX9 Promotes Inflammatory and Immune Responses of Dental Pulp." dans: Journal of endodontics, Vol. 44, Issue 5, pp. 792-799, (2018) (PubMed).

Zhu, Liu, Zhang, Pan, Lin, Li, Wang, Yang, Yan, Gong: "Circulating and Adipose Tissue mRNA Levels of Zinc-α2-Glycoprotein, Leptin, High-Molecular-Weight Adiponectin, and Tumor Necrosis Factor-Alpha in Colorectal Cancer Patients With or Without Obesity." dans: Frontiers in endocrinology, Vol. 9, pp. 190, (2018) (PubMed).

Waldner, Dassati, Redl, Smania, Gandolfi: "Apolipoprotein D Concentration in Human Plasma during Aging and in Parkinson's Disease: A Cross-Sectional Study." dans: Parkinson's disease, Vol. 2018, pp. 3751516, (2018) (PubMed).

Détail du antigène

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Autre désignation: TNFa

Sujet: DIF, TNF-A, TNFSF2, Cachectin, Tumor Necrosis Factor Ligand Superfamily Member 2

UniProt: P01375

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome