Insulin Kit ELISA

N° du produit ABIN415467

Le kit ELISA Insulin (ABIN415467) Souris est un kit ELISA Colorimetric conçu pour quantifier Souris Insulin. Ce kit ELISA a été cité dans 32 publications.

Aperçu rapide pour Insulin Kit ELISA (ABIN415467)

Antigène: Insulin (INS)

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Gamme de detection: 6.17 pg/mL - 500 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Détail du produit Insulin Kit ELISA

Seuil minimal de détection: 6.17 pg/mL

Fonction: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of insulin in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates.

We offer validation data (WB) for the kit components. So you can be sure to order a reliable ELISA kit product composed of high quality reagents.

Analytical Method: Quantitative

Specificité:

This assay has high sensitivity and excellent specificity for detection of Insulin (INS).
No significant cross-reactivity or interference between Insulin (INS) and analogues was observed.

Réactivité croisée (Details): No significant cross-reactivity or interference between Insulin (INS) and analogues was observed.

Sensibilité: < 2.65 pg/mL

Ingrédients:

• Pre-coated, 96-well strip plate
• Plate sealer
• Standard
• Standard Diluent
• Detection Reagent A
• Assay Diluent A
• Detection Reagent B
• Assay Diluent B
• TMB Substrate
• Stop Solution
• Wash Buffer (30X)
• Instruction manual

Matériel non inclus:

• Microplate reader with 450 nm filter.
• Precision single or multi-channel pipettes and disposable tips.
• Eppendorf Tubes for diluting samples.
• Deionized or distilled water.
• Absorbent paper for blotting the microtiter plate.
• Container for Wash Solution

Information d'application

Indications d'application:

• Limited by the current condition and scientific technology, we cannot completely conduct the comprehensive identification and analysis on the raw material provided by suppliers. So there might be some qualitative and technical risks to use the kit.
• The final experimental results will be closely related to validity of the products, operation skills of the end users and the experimental environments. Please make sure that sufficient samples are available.
• Kits from different batches may be a little different in detection range, sensitivity and color developing time.
• Do not mix or substitute reagents from one kit lot to another. Use only the reagents supplied by manufacturer.
• Protect all reagents from strong light during storage and incubation. All the bottle caps of reagents should be covered tightly to prevent the evaporation and contamination of microorganism.
• There may be some foggy substance in the wells when the plate is opened at the first time. It will not have any effect on the final assay results. Do not remove microtiter plate from the storage bag until needed.
• Wrong operations during the reagents preparation and loading, as well as incorrect parameter setting for the plate reader may lead to incorrect results. A microplate plate reader with a bandwidth of 10nm or less and an optical density range of 0-3 O.D. or greater at 450 ± 10nm wavelength is acceptable for use in absorbance measurement. Please read the instruction carefully and adjust the instrument prior to the experiment.
• Even the same operator might get different results in two separate experiments. In order to get better reproducible results, the operation of every step in the assay should be controlled. Furthermore, a preliminary experiment before assay for each batch is recommended.
• Each kit has been strictly passed Q.C test. However, results from end users might be inconsistent with our in-house data due to some unexpected transportation conditions or different lab equipments. Intra-assay variance among kits from different batches might arise from above factors, too.
• Kits from different manufacturers for the same item might produce different results, since we have not compared our products with other manufacturers.

Commentaires:

Information on standard material:
The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

Information on reagents:
The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

Information on antibodies:
The provided antibodies and their host vary in different kits.

Volume d'échantillon: 50 μL

Durée du test: 2 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

Précision du teste:

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin (INS) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin (INS) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Précaution d'utilisation: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Conseil sur la manipulation: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Stock: 4 °C

Stockage commentaire:

• For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
• For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
  Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
• For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.

Date de péremption: 6 months

Références pour Insulin Kit ELISA (ABIN415467)

Zhu, Mao, Wu, Yu, Cheng, Xiao, Ye, Linhardt, Orfila, Chen: "Highly Branched RG-I Domain Enrichment Is Indispensable for Pectin Mitigating against High-Fat Diet-Induced Obesity." dans: Journal of agricultural and food chemistry, Vol. 68, Issue 32, pp. 8688-8701, (2021) (PubMed).

Fang, He, Yu, Shi, Zhu, Zhang, Bo: "Treatment with celastrol protects against obesity through suppression of galanin-induced fat intake and activation of PGC-1α/GLUT4 axis-mediated glucose consumption." dans: Biochimica et biophysica acta. Molecular basis of disease, Vol. 1865, Issue 6, pp. 1341-1350, (2020) (PubMed).

Shi, Yao, Lian, Liu, Liu, Yang, Yang, Li: "Regulating glycolysis, the TLR4 signal pathway and expression of RBM3 in mouse liver in response to acute cold exposure." dans: Stress (Amsterdam, Netherlands), Vol. 22, Issue 3, pp. 366-376, (2020) (PubMed).

Wang, Wu, Diao, Li, Sun, Xiao: "Huperzine A ameliorates obesity-related cognitive performance impairments involving neuronal insulin signaling pathway in mice." dans: Acta pharmacologica Sinica, Vol. 41, Issue 2, pp. 145-153, (2020) (PubMed).

Zhao, Cai, Jiang, Li, Zhong, Zhang, Feng: "Glycerol-Monolaurate-Mediated Attenuation of Metabolic Syndrome is Associated with the Modulation of Gut Microbiota in High-Fat-Diet-Fed Mice." dans: Molecular nutrition & food research, Vol. 63, Issue 18, pp. e1801417, (2020) (PubMed).

Fang, Xia, Xu, Wu, Ma, Zhao, Gu: "Isoflurane aggravates peripheral and central insulin resistance in high-fat diet/streptozocin-induced type 2 diabetic mice." dans: Brain research, Vol. 1727, pp. 146511, (2020) (PubMed).

Wang, Le, Lu, Zhao, Dou, Zhang: "Triphenyl phosphate causes a sexually dimorphic metabolism dysfunction associated with disordered adiponectin receptors in pubertal mice." dans: Journal of hazardous materials, Vol. 388, pp. 121732, (2020) (PubMed).

Chen, Lin, Xu, Zhang, Cai, Shao, Chen, Chen, Weng: "Liraglutide improved inflammation via mediating IL-23/Th-17 pathway in obese diabetic mice with psoriasiform skin." dans: The Journal of dermatological treatment, pp. 1-7, (2020) (PubMed).

Hatem-Vaquero, Griera, Garcia-Ayuso, Campillo, Bohorquez, Calleros, Rodriguez-Puyol, Rodriguez-Puyol, de Frutos: "Integrin Linked Kinase (ILK) Downregulation as an Early Event During the Development of Metabolic Alterations in a Short-Term High Fat Diet Mice Model." dans: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 54, Issue 1, pp. 71-87, (2020) (PubMed).

Wang, Dilidaxi, Wu, Sailike, Sun, Nabi: "Composite probiotics alleviate type 2 diabetes by regulating intestinal microbiota and inducing GLP-1 secretion in db/db mice." dans: Biomedicine & pharmacotherapy, Vol. 125, pp. 109914, (2020) (PubMed).

Liu, Zhang, Yuan, Song, Zhang, Lin, Li, Sheng, Ma, Lv, Gao, Dong: "Hyperbaric Oxygen Ameliorates Insulin Sensitivity by Increasing GLUT4 Expression in Skeletal Muscle and Stimulating UCP1 in Brown Adipose Tissue in T2DM Mice." dans: Frontiers in endocrinology, Vol. 11, pp. 32, (2020) (PubMed).

Bi, Zheng, Wang, Zhou: "Mice with nucleus tractus solitarius injury induced by chronic restraint stress present impaired ability to raise blood glucose and glucagon levels when blood glucose levels plummet." dans: Endocrine journal, Vol. 67, Issue 7, pp. 771-783, (2020) (PubMed).

Liu, Cui, Gao, Xue, Xu, Chang, Jiang, Liang: "Aplysin Retards Pancreatic Necrosis and Inflammatory Responses in NOD Mice by Stabilizing Intestinal Barriers and Regulating Gut Microbial Composition." dans: Mediators of inflammation, Vol. 2020, pp. 1280130, (2020) (PubMed).

Yang, Zhang, Zhang, Dan, Zhou, Wang, Li, Zhou, Yang, Xie: "Insulin Promotes Corneal Nerve Repair and Wound Healing in Type 1 Diabetic Mice by Enhancing Wnt/β-Catenin Signaling." dans: The American journal of pathology, Vol. 190, Issue 11, pp. 2237-2250, (2020) (PubMed).

Guo, Chang, Yu, Xu, Yi, Cao: "Adiponectin treatment improves insulin resistance in mice by regulating the expression of the mitochondrial-derived peptide MOTS-c and its response to exercise via APPL1-SIRT1-PGC-1α." dans: Diabetologia, Vol. 63, Issue 12, pp. 2675-2688, (2020) (PubMed).

Deng, Li, Ren, He, Jia, Liu, Zhang, Gan, Liu: "Thioredoxin-interacting protein: a critical link between autophagy disorders and pancreatic β-cell dysfunction." dans: Endocrine, Vol. 70, Issue 3, pp. 526-537, (2020) (PubMed).

Yang, Chen, Li, Hui, Gao: "Geniposide and Gentiopicroside Suppress Hepatic Gluconeogenesis via Regulation of AKT-FOXO1 Pathway." dans: Archives of medical research, Vol. 49, Issue 5, pp. 314-322, (2019) (PubMed).

Liu, Yao, Shi, Liu, Lian, Yang, Yang, Li: "Effects of Cold-inducible RNA-binding Protein (CIRP) on Liver Glycolysis during Acute Cold Exposure in C57BL/6 Mice." dans: International journal of molecular sciences, Vol. 20, Issue 6, (2019) (PubMed).

Niu, Yang, Yang: "Synthesis and anti-diabetic activity of new N,N-dimethylphenylenediamine-derivatized nitrilotriacetic acid vanadyl complexes." dans: Journal of inorganic biochemistry, Vol. 177, pp. 291-299, (2018) (PubMed).

Chen, Yang, Nie, Song, Gu: "Effects of Bariatric Surgery on Change of Brown Adipocyte Tissue and Energy Metabolism in Obese Mice." dans: Obesity surgery, Vol. 28, Issue 3, pp. 820-830, (2018) (PubMed).

Détail du antigène

Antigène: Insulin (INS)

Autre désignation: INS

UniProt: P01326

Pathways: Signalisation NF-kappaB, Signalisation RTK, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation