COL1A1 Kit ELISA

N° du produit ABIN454769

Détail du produit COL1A1 Kit ELISA

Antigène: COL1A1 (Collagen, Type I, alpha 1 (COL1A1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.469-30 ng/mL

Seuil minimal de détection: 0.469 ng/mL

Application: ELISA

Fonction: This immunoassay kit allows for the in vitro quantitative determination of human Col ? concentrations in serum, plasma and other biological fluids.

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This assay recognizes recombinant and natural human Col ?.

Réactivité croisée (Details): No significant cross-reactivity or interference was observed.

Sensibilité: < 0.117 ng/mL
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.

Attributs du produit: Homo sapiens,Human,Collagen alpha-1(I) chain,Alpha-1 type I collagen,COL1A1

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Col Ⅰ. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Col Ⅰ. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Col Ⅰ, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of Col Ⅰ in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Stockage commentaire: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Détail du antigène

Antigène: COL1A1 (Collagen, Type I, alpha 1 (COL1A1))

Autre désignation: COL1A1 (COL1A1 Produits)

Synonymes: OI4 Kit ELISA, Col1a-1 Kit ELISA, Cola-1 Kit ELISA, Cola1 Kit ELISA, Mov-13 Kit ELISA, Mov13 Kit ELISA, COLIA1 Kit ELISA, col1a1 Kit ELISA, MGC52532 Kit ELISA, COL1A1 Kit ELISA, COL1A2 Kit ELISA, alpha1(I) Kit ELISA, cb21 Kit ELISA, sb:cb21 Kit ELISA, sb:cb384 Kit ELISA, wu:fa99c12 Kit ELISA, wu:fb02c06 Kit ELISA, alpha3(I) Kit ELISA, col1a3 Kit ELISA, hm:zeh0348 Kit ELISA, wu:fa95h05 Kit ELISA, wu:fd02a10 Kit ELISA, wu:fj59a10 Kit ELISA, collagen type I alpha 1 chain Kit ELISA, collagen, type I, alpha 1 Kit ELISA, collagen, type I, alpha 1 S homeolog Kit ELISA, collagen alpha-1(I) chain Kit ELISA, collagen type I alpha 2 chain Kit ELISA, collagen, type I, alpha 1a Kit ELISA, collagen 1a1 Kit ELISA, collagen, type I, alpha 1b Kit ELISA, COL1A1 Kit ELISA, Col1a1 Kit ELISA, col1a1.S Kit ELISA, LOC397571 Kit ELISA, COL1A2 Kit ELISA, col1a1a Kit ELISA, col1a1 Kit ELISA, col1a1b Kit ELISA

Sujet: Collagen is an inert, rigid protein found predominantly in skin, ligaments, bones and teeth. Its most distinctive attribute, essential to a transmitter of mechanical force, is inelasticity. Its fundamental structural unit is tropo-collagen, a molecular rod about 2600Å in length and 15Å in diameter and 300,000 molecular weight. In tendons these macromolecules, grouped as collagen fibrils, run parallel to the axis. In skin the fibrils are interlaced and branched. Type 1 collagen is the most abundant protein in the connective tissues, especially in tendon, skin and bone.

Pathways: Sensory Perception of Sound, Autophagy, Growth Factor Binding