BMPR1B Kit ELISA

N° du produit ABIN4881803

Détail du produit BMPR1B Kit ELISA

Antigène: BMPR1B (Bone Morphogenetic Protein Receptor, Type IB (BMPR1B))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human ALK-6/BMPR-IB ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human ALK-6.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: BMPR1B (Bone Morphogenetic Protein Receptor, Type IB (BMPR1B))

Autre désignation: ALK-6 (BMPR1B Produits)

Synonymes: ALK6 Kit ELISA, BMPR-IB Kit ELISA, FecB Kit ELISA, alk-6 Kit ELISA, alk6 Kit ELISA, cdw293 Kit ELISA, alk6b Kit ELISA, zgc:172219 Kit ELISA, BMPR1B Kit ELISA, AI385617 Kit ELISA, ALK-6 Kit ELISA, AV355320 Kit ELISA, Acvrlk6 Kit ELISA, Alk6 Kit ELISA, BMPR-1B Kit ELISA, CFK-43a Kit ELISA, SKR6 Kit ELISA, BR1b Kit ELISA, alk6tr Kit ELISA, bmpr1b Kit ELISA, zALK-6 Kit ELISA, zgc:92220 Kit ELISA, CDw293 Kit ELISA, BMP15 Kit ELISA, BMPRIB Kit ELISA, RPK-1 Kit ELISA, bone morphogenetic protein receptor type 1B Kit ELISA, bone morphogenetic protein receptor, type IBb Kit ELISA, bone morphogenetic protein receptor, type 1B Kit ELISA, bone morphogenetic protein receptor, type IBa Kit ELISA, BMPR1B Kit ELISA, bmpr1b Kit ELISA, bmpr1bb Kit ELISA, Bmpr1b Kit ELISA, bmpr1ba Kit ELISA

ID gène: 658

UniProt: O00238