Cytokeratin 18 Kit ELISA

N° du produit ABIN4882548

Détail du produit Cytokeratin 18 Kit ELISA

Antigène: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.164-40 ng/mL

Seuil minimal de détection: 0.164 ng/mL

Application: ELISA

Fonction: Human Cytokeratin 18/KRT18 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human Cytokeratin 18.

Sensibilité: 160 pg/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples2 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Autre désignation: Cytokeratin 18 (KRT18 Produits)

Synonymes: CK18 Kit ELISA, K18 Kit ELISA, Krt1-18 Kit ELISA, CYK18 Kit ELISA, DreK18 Kit ELISA, cb83 Kit ELISA, dapk1 Kit ELISA, sb:cb83 Kit ELISA, wu:fa13f02 Kit ELISA, wu:fb36b04 Kit ELISA, krt18 Kit ELISA, MGC64569 Kit ELISA, MGC75922 Kit ELISA, KRT18 Kit ELISA, CK-18-A Kit ELISA, K18-A Kit ELISA, NC-11 Kit ELISA, cyk18 Kit ELISA, k18 Kit ELISA, krt18-a Kit ELISA, krt18-b Kit ELISA, krt18a Kit ELISA, xkendob Kit ELISA, keratin 18 Kit ELISA, keratin 18, type I L homeolog Kit ELISA, keratin 18, type I Kit ELISA, keratin, type I cytoskeletal 18 Kit ELISA, K18, simple type I keratin Kit ELISA, Keratin, type I cytoskeletal 18 Kit ELISA, keratin 18, type I S homeolog Kit ELISA, Krt18 Kit ELISA, KRT18 Kit ELISA, krt18 Kit ELISA, krt18.L Kit ELISA, LOC700569 Kit ELISA, LOC100136778 Kit ELISA, k1c18 Kit ELISA, LOC100008885 Kit ELISA, krt18.S Kit ELISA

ID gène: 3875

UniProt: P05783

Pathways: Apoptose, Caspase Cascade in Apoptosis