S100A1 Kit ELISA
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- Antigène Voir toutes S100A1 Kits ELISA
- S100A1 (S100 Calcium Binding Protein A1 (S100A1))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 0.82-200 ng/mL
- Seuil minimal de détection
- 0.82 ng/mL
- Application
- ELISA
- Fonction
- Human S100 A1 ELISA Kit for serum, plasma, and cell culture supernatant samples.
- Type d'échantillon
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- Specificité
- This ELISA antibody pair detects human S100A1. Other species not determined.
- Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Indications d'application
- Recommended Dilution for serum and plasma samples2 fold
- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Stock
- -20 °C
- Stockage commentaire
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Date de péremption
- 6 months
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- Antigène Voir toutes S100A1 Kits ELISA
- S100A1 (S100 Calcium Binding Protein A1 (S100A1))
- Autre désignation
- S100 A1 (S100A1 Produits)
- Synonymes
- S100 Kit ELISA, S100-alpha Kit ELISA, S100A Kit ELISA, S100A1 Kit ELISA, DKFZp468K0511 Kit ELISA, AI266795 Kit ELISA, S100a Kit ELISA, S100 calcium binding protein A1 Kit ELISA, S100A1 Kit ELISA, S100a1 Kit ELISA, s100a1 Kit ELISA
- ID gène
- 6271
- UniProt
- P23297
- Pathways
- Regulation of Muscle Cell Differentiation, Toll-Like Receptors Cascades, S100 Proteins
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