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SEMA6D Kit ELISA
SEMA6D
Reactivité: Humain
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN4884466
Détail du produit SEMA6D Kit ELISA
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Antigène
Tous les produits SEMA6D
SEMA6D
(Sema Domain, Transmembrane Domain (TM), and Cytoplasmic Domain, (Semaphorin) 6D (SEMA6D))
Reactivité
Humain
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Fonction
Human Semaphorin 6D ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
Type d'échantillon
Plasma, Cell Culture Supernatant, Serum
Analytical Method
Quantitative
Specificité
This ELISA antibody pair recognizes Human Semaphorin 6D.
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Alternatives
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Information d'application
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Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Tous les produits SEMA6D
SEMA6D
(Sema Domain, Transmembrane Domain (TM), and Cytoplasmic Domain, (Semaphorin) 6D (SEMA6D))
Autre désignation
Semaphorin 6D (SEMA6D Produits )
Synonymes
1110067B02Rik Kit ELISA, AA409156 Kit ELISA, D330011G23 Kit ELISA, Sema6D-1 Kit ELISA, Sema6D-2 Kit ELISA, Sema6D-4 Kit ELISA, Sema6D-5 Kit ELISA, Sema6D-6 Kit ELISA, mKIAA1479 Kit ELISA, wu:fi06a12 Kit ELISA, wu:fi76a10 Kit ELISA, zgc:56170 Kit ELISA, semaphorin 6D Kit ELISA, sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D Kit ELISA, semaphorin 6D L homeolog Kit ELISA, SEMA6D Kit ELISA, Sema6d Kit ELISA, sema6d Kit ELISA, sema6d.L Kit ELISA
ID gène
80031
UniProt
Q8NFY4
Pathways
Smooth Muscle Cell Migration
Vus récemment
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