SULT2A1 Kit ELISA

N° du produit ABIN4884692

Détail du produit SULT2A1 Kit ELISA

Antigène: SULT2A1 (Sulfotransferase Family, Cytosolic, 2A, Dehydroepiandrosterone (DHEA)-Preferring, Member 1 (SULT2A1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human SULT2A1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: SULT2A1 (Sulfotransferase Family, Cytosolic, 2A, Dehydroepiandrosterone (DHEA)-Preferring, Member 1 (SULT2A1))

Autre désignation: SULT2A1 (SULT2A1 Produits)

Synonymes: dhea-st Kit ELISA, dheas Kit ELISA, st2 Kit ELISA, st2a1 Kit ELISA, st2a3 Kit ELISA, sta Kit ELISA, std Kit ELISA, SULT2A1 Kit ELISA, ST2A1 Kit ELISA, Std Kit ELISA, Sth1 Kit ELISA, mSTa1 Kit ELISA, STa Kit ELISA, Smp-2 Kit ELISA, St2 Kit ELISA, St2a1 Kit ELISA, Sth2 Kit ELISA, DHEA-ST Kit ELISA, DHEAS Kit ELISA, HST Kit ELISA, ST2 Kit ELISA, ST2A3 Kit ELISA, STD Kit ELISA, hSTa Kit ELISA, sulfotransferase family 2A member 1 Kit ELISA, sulfotransferase family 2A member 1 L homeolog Kit ELISA, alcohol sulfotransferase Kit ELISA, sulfotransferase family 2A, dehydroepiandrosterone (DHEA)-preferring, member 1 Kit ELISA, SULT2A1 Kit ELISA, sult2a1.L Kit ELISA, PCC8801_2155 Kit ELISA, Cyan8802_2217 Kit ELISA, NAL212_1203 Kit ELISA, sult2a1 Kit ELISA, Sult2a1 Kit ELISA

ID gène: 6822

UniProt: Q06520

Pathways: Steroid Hormone Biosynthesis, Regulation of Lipid Metabolism by PPARalpha, Monocarboxylic Acid Catabolic Process