VHL Kit ELISA

N° du produit ABIN4885037

Détail du produit VHL Kit ELISA

Antigène: VHL (Von Hippel-Lindau Tumor Suppressor, E3 Ubiquitin Protein Ligase (VHL))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human VHL ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: VHL (Von Hippel-Lindau Tumor Suppressor, E3 Ubiquitin Protein Ligase (VHL))

Autre désignation: VHL (VHL Produits)

Synonymes: HRCA1 Kit ELISA, RCA1 Kit ELISA, VHL1 Kit ELISA, pVHL Kit ELISA, BcDNA:RH61560 Kit ELISA, CG13221 Kit ELISA, DVhl Kit ELISA, Dmel\\CG13221 Kit ELISA, Dvhl Kit ELISA, VHL Kit ELISA, d-VHL Kit ELISA, d-vhl Kit ELISA, dVHL Kit ELISA, dmVHL Kit ELISA, vhl Kit ELISA, rca1 Kit ELISA, vhl1 Kit ELISA, hrca1 Kit ELISA, zgc:158722 Kit ELISA, Vhlh Kit ELISA, von Hippel-Lindau tumor suppressor Kit ELISA, von Hippel-Lindau Kit ELISA, von Hippel-Lindau tumor suppressor, E3 ubiquitin protein ligase Kit ELISA, von Hippel-Lindau disease tumor suppressor Kit ELISA, VHL Kit ELISA, Vhl Kit ELISA, vhl Kit ELISA, CpipJ_CPIJ009992 Kit ELISA

ID gène: 7428

UniProt: P40337

Pathways: Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Ubiquitin Proteasome Pathway