WNT4 Kit ELISA

N° du produit ABIN4885058

Détail du produit WNT4 Kit ELISA

Antigène: WNT4 (Wingless-Type MMTV Integration Site Family, Member 4 (WNT4))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.22-300 ng/mL

Seuil minimal de détection: 1.22 ng/mL

Application: ELISA

Fonction: Human Wnt-4 ELISA Kit for serum, plasma, and cell culture supernatant samples.

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: This ELISA antibody pair detects human Wnt-4. Other species not determined.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples2 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: WNT4 (Wingless-Type MMTV Integration Site Family, Member 4 (WNT4))

Autre désignation: Wnt-4 (WNT4 Produits)

Synonymes: CG4698 Kit ELISA, DWnt-4 Kit ELISA, DWnt4 Kit ELISA, Dm DWnt4 Kit ELISA, Dmel\\CG4698 Kit ELISA, Dwnt4 Kit ELISA, Wnt Kit ELISA, Wnt-4 Kit ELISA, anon-EST:Liang-2.4 Kit ELISA, clone 2.4 Kit ELISA, wnt-4 Kit ELISA, wnt4 Kit ELISA, SERKAL Kit ELISA, WNT-4 Kit ELISA, Xwnt4 Kit ELISA, xwnt-4 Kit ELISA, zgc:136737 Kit ELISA, Wnt family member 4 Kit ELISA, Wnt oncogene analog 4 Kit ELISA, wingless-type MMTV integration site family, member 4 Kit ELISA, wingless-type MMTV integration site family member 4 S homeolog Kit ELISA, wingless-type MMTV integration site family, member 4a Kit ELISA, WNT4 Kit ELISA, Wnt4 Kit ELISA, wnt4.S Kit ELISA, wnt4a Kit ELISA

ID gène: 54361

UniProt: P56705

Pathways: Signalisation WNT, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Cell-Cell Junction Organization, Tube Formation