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AGT Kit ELISA
AGT
Reactivité: Humain
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN4885084
Détail du produit AGT Kit ELISA
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Antigène
Voir toutes AGT Kits ELISA
AGT
(Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT))
Reactivité
Toutes les réactivités sur AGT Kits ELISA
Humain
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Fonction
Custom Human Angiotensinogen (SERPINA8) SpeedELISA Kit.
Marque
SpeedELISA
Type d'échantillon
Plasma, Cell Culture Supernatant, Serum
Analytical Method
Quantitative
Attributs du produit
Compressed workflow with only one wash step and three incubation steps 3 hours total processing time Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Streptavidin-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Capture Antibody HRP Conjugated Detection Antibody TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Information d'application
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Volume d'échantillon
100 μL
Durée du test
3 h
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 70 μL of the Antibody Cocktail to each well. Incubate 10 minutes at RT. Add 50 μL of sample or standard to each well. Incubate 2 h at RT. Wash Wells Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes AGT Kits ELISA
AGT
(Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT))
Autre désignation
Angiotensinogen (AGT Produits )
Synonymes
ANHU Kit ELISA, SERPINA8 Kit ELISA, AI265500 Kit ELISA, AngI Kit ELISA, AngII Kit ELISA, Aogen Kit ELISA, Serpina8 Kit ELISA, ANRT Kit ELISA, Ang Kit ELISA, PAT Kit ELISA, wu:fb62f06 Kit ELISA, wu:fj87b02 Kit ELISA, zgc:111892 Kit ELISA, AGT Kit ELISA, angt Kit ELISA, ANGT Kit ELISA, angiotensinogen Kit ELISA, angiotensinogen (serpin peptidase inhibitor, clade A, member 8) Kit ELISA, AGT Kit ELISA, Agt Kit ELISA, agt Kit ELISA
ID gène
183
UniProt
P01019
Pathways
Signalistation JAK/STAT , ACE Inhibitor Pathway , EGFR Signaling Pathway , Peptide Hormone Metabolism , Regulation of Systemic Arterial Blood Pressure by Hormones , Regulation of Lipid Metabolism by PPARalpha , Protein targeting to Nucleus , Feeding Behaviour , Monocarboxylic Acid Catabolic Process , Dicarboxylic Acid Transport , Positive Regulation of Response to DNA Damage Stimulus , Regulation of long-term Neuronal Synaptic Plasticity
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